U937和K562细胞株对登革病毒2型感染性的研究  

Studyondengue-2virusinfectionwithU937andK562cells

作　　者：李胜华[1,2,3,4,5,6] 詹轶群[1,2,3,4,5,6] 郭庆福[1,2,3,4,5,6] 周洁 戴庆华[1,2,3,4,5,6] 高杰英 曹军田[1,2,3,4,5,6] 李予川[1,2,3,4,5,6] 孔惟惟[1,2,3,4,5,6] 

机构地区：[1]北京军事医学科学院微生物流行病研究所 [2]中国预防医学科学院病毒学研究所 [3]河南省新乡医学院 [4]江苏省南京市传染病医院 [5]福建省卫生防疫站 [6]南京大学医学烷

出　　处：《中华实验和临床病毒学杂志》1996年第3期243-246,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：运用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）、酶抗体染色法结合电镜观察和病毒滴定。初步研究了Ｕ９３７和Ｋ５６２细胞株对登革病毒２型感染性。结果显示两株细胞均可被感染，Ｕ９３７和Ｋ５６２细胞的感染率分别为１２％和３３．３％。serumsamples(including89pairedseraand70singlesera)werecollectedfrompatientsclinicallydiag-nosedasJapaneseEncephalitis(JE)andViralEncephalitisinSummer-Autumnseasonof1994.inHenan,Nanjing,andFu-jianProvince.Risingofcoltivirusantibodywithfourormorethanfourfoldswasdemonstratedin7casesamong89testedpairedserabyusingELISAmethod.Of159serumspecimens,50withcoltivirusantlbodypositivewerefound,andtheposi-tiveratewas31.5%.ColtivirusandJEvirusantibodiesweretestedbyusingELISAandReversePassiveHemagglutinationInhibition(RPHI)test,respectively.Theresultsfoundthattherewerefourkindsofantilodyresponsepattem:51and19of159sample(32.1%* and11.9%)wereJEandcoltivirusantilbodypositivealone,respectively.31of159(19.5%)werebothJEvirusandcoltivirusantitodypositive.58of159(36.5%* )werebothJEvizusandcoltivirusantibodynegative.TheresultsindicatedthatcoltivirusisanimportantagentcausingencephalitisinChinabesidesJEvirus,andtheprevalentseseonOfcoltivirusisthesameasthatofJEvirus.

关 键 词：登革病毒2型 聚合酶链反应 细胞株 

分 类 号：R373.33[医药卫生—病原生物学]