微乳液电动色谱测定油-水分配系数的改进方法研究  

Measurement of Oil-Water Partition Coefficients by Improved Microemulsion Electrokinetic Chromatography

作　　者：蒋雪梅[1] 魏为力[1] 姜军坡[1] 石开云[2] 穆小静[1] 陈志涛[2] 夏之宁[1] 

机构地区：[1]重庆大学化学化工学院药学系 [2]重庆大学生物力学与组织工程教育部重点实验室,重庆400044

出　　处：《高等学校化学学报》2006年第8期1424-1429,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20375051)资助

摘　　要：通过考察微乳液电动色谱(MEEKC)中化合物浓度和高电场对其迁移行为的影响,探讨了化合物分配于微乳后改变微乳内相性质的原因,以及电泳高电场致使微乳液性质变化的机理.在此基础上建立了一种测定化合物油-水分配系数的改进型MEEKC方法.将此改进方法应用于烷基苯化合物之油-水分配系数的测定,其测定值与文献参考值平均相差0.07个对数单位,准确度较现行MEEKC方法有了明显提高.Microemulsion electrokinetic chromatography（MEEKC） was used as a powerful method for the indirect measurement of oil-water partition coefficients of organic compounds. Considering the problems of determining oil-water partition coefficients by conventional MEEKC, the concentration of solute and high electricfield having impact on the migration behavior of compound was studied, the reason and mechanism of which were also studied. Moreover, an improved method of MEEKC （I-MEEKC） that can be used to solve the problems found was developed. Applying this improved method to determining oil-water partition coefficients for compounds, such as alkyl-benzene： benzene, toluene and ethyl benzene, good resuh was obtained： the average error between oil-water partition coefficients estimated by using improved MEEKC and literature values published was 0. 07 logarithm units. In this work not only a new method was established to measure oil-water partition coefficients with MEEKC, but also the improved MEEKC is developed further and become more reliable and more accurate method for qualitative analysis.

关 键 词：MEEKC 改进方法 油-水分配系数 高电场 

分 类 号：O657[理学—分析化学]