电喷雾解吸电离质谱法用于临床尿样的直接分析  

Desorption Electrospray Ionization Mass Spectrometry for Direct Analysis of Clinic Urine Samples

作　　者：陈焕文[1] 李明[1] 周建光[1] 费强[1] 姜杰[1] 金钦汉[1] 张天幕[2] 张燮[3] 

机构地区：[1]吉林大学化学学院吉林省光谱仪器分析仪器工程技术研究中心,长春130021 [2]吉林大学中日联谊医院,长春130039 [3]东华理工学院应用化学系,抚州344000

出　　处：《高等学校化学学报》2006年第8期1439-1442,共4页Chemical Journal of Chinese Universities

基　　金：国家科技攻关项目(批准号:2004BA210A04)资助

摘　　要：将电喷雾解吸电离质谱(DESI-MS)用于临床尿样的分析,优化了电喷雾溶剂流速、电喷雾电压和喷雾锥距离等重要参数.采用普通滤纸作为样品载体,在不需要样品预处理的前提下同时快速测定了临床尿样中的钾、钠、尿素、尿酸、丙酮酸和肌苷等多种成分,并对各种成分的主要离子进行了串联质谱鉴定.DESI-MS在进行多组分同时测定时不需要进行样品预处理,缩短了测定时间,单个样品的分析时间不到1 m in.同时,采用内标法对所测定组分进行了半定量分析.DESI-MS was applied to direct simultaneous detection of multiple components of clinic urine sampies without any sample pretreatment. Under the optimized working conditions, such as ESI infusion rate, ESI high voltage, incident angle, collective angle, tube lens voltage, etc. , for both DESI and LTQ mass spectro- meter, the main components of urine including urea, pyruvic acid, creatinine, oxalic acid, alloxane, menadione were detected as the protonated molecules in positive detection mode using methanol-water（ volume ratio 1：1 ） as the spray solvent in the low mass range（ 15--200 amu）. Sodium and potassium were also detected but mainly in the form of clusters with urea and creatinine. In each case, the corresponding ions were isolated in the ion trap mass analyzer and underwent a collision-induced dissociation process, which yielded characteristic fragments in tandem mass spectra and resulted in confident validation of the analytical results. The average time for single sample analysis was less than 1 min since it required no sample pretreatment. Furthermore, the semi-quantitative analysis of the components was also demonstrated by adding serine as an internal standard into the samples.

关 键 词：电喷雾电离质谱 尿样 串联质谱 同时测定 

分 类 号：O657[理学—分析化学]