化学发光磁酶免疫法检测O157∶H7大肠埃希菌  

Determination of Escherichia Coli O157∶H7 in Food Based on Chemiluminescent Magnetic Enzyme-linked Immunoassay

作　　者：朱广华[1] 贺艳峰[1] 郭小英[2] 刘和平[3] 

机构地区：[1]东南大学公共卫生学院,南京210009 [2]东南大学生物科学与医学工程系分子电子学国家重点实验室,南京210096 [3]江苏省疾病预防控制中心,南京210009

出　　处：《高等学校化学学报》2006年第8期1453-1455,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:60121101;60223002);国家"八六三"计划项目(批准号:2002AA2Z2041;2004AA302070)资助

摘　　要：A high sensitive chemiluminescent magnetic enzyme-linked immunoassay method was established for Escherichia coli O157∶H7 determination.The bacterium antibody was labeled by alkaline phosphatase（ALP） that catalyzed the decomposing of substrate 3-（2-spiroadamantane）-4-methoxy-4（3-phosphoryloxy）phenyl-1,2-dioxetane（AMPPD） to give the light emission.The sensitivity of the method is 8.5×104 Cell/mL with a linear range of 1.0×105—5.0×107 Cell/mL.The intra- and inter-assay CVs are 14.8% and 20.0% in pork samples, respectively.The correlation coefficient of present and the standard plate counting method is 0.981.The experiments with the spiked samples show that this method has great potential to be applied to（detecting） the concentration of Escherichia coli O157∶H7 in a variety of samples.A high sensitive chemiluminescent magnetic enzyme-linked immunoassay method was established for Escherichia coli 0157：H7 determination. The bacterium antibody was labeled by alkaline phosphatase （ALP） that catalyzed the decomposing of substrate 3-（ 2-spiroadamantane ） -4-methoxy-4 （3-phosphoryloxy） phenyl-1,2-dioxetane（AMPPD） to give the light emission. The sensitivity of the method is 8.5 ×10^4 Cell/mL with a linear range of 1.0 × 10^5-5.0 × 10^7 Cell/mL. The intra- and inter-assay CVs are 14. 8% and 20. 0% in pork samples, respectively. The correlation coefficient of present and the standard plate counting method is 0. 981. The experiments with the spiked samples show that this method has great potential to be applied to detecting the concentration of Escherichia coli 0157：H7 in a variety of samples.

关 键 词：免疫分析 化学发光 磁性微球 大肠埃希菌 

分 类 号：O657.39[理学—分析化学]