一种新型独特芋螺毒素的分离与结构鉴定  

Isolation and Structure Characterization of a New T-Superfamily Conotoxin from Conus Caracteristicus

作　　者：刘尚义[1] 曹瑛[1] 赵听友[1] 代先东[1] 钟明鼐[1] 范崇旭[1] 陈冀胜[1] 

机构地区：[1]解放军防化研究院第四研究所,北京102205

出　　处：《高等学校化学学报》2006年第8期1482-1484,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20272083)资助

摘　　要：Cone snails are tropical marine predatory molluscs,and there is an unprecedented variety of neuropharmacologically active peptide.Over seventy living species of cone snails are distributed in China.The ve-nom of Conus caracteristicus collected from South China Sea was studied in this work.A new conotoxin,cr5a,was isolated from the venom through Sephadex G-25 chromatography and HPLC,and then was identified by（amino） acid analysis,mass spectrometry,N-terminal sequence.The primary structure of cr5a was CCKFQFLNFCCNE,it shared a conserved T-superfamily of conotoxins arrangement of cysteine residues（CC-CC）.Total chemical synthesis of the two possible disulfide linkages of cr5a was achieved,and the result of HPLC co-elution showed that cr5a was a tridecapeptide with a disulfide connectivity of Cys1-Cys10, Cys2-Cys11.So cr5a is a new member of the T-superfamily conotoxins.Cone snails are tropical marine predatory molluscs, and there is an unprecedented variety of neuropharmacologically active peptide. Over seventy living species of cone snails are distributed in China. The venom of Conus caracteristicus collected from South China Sea was studied in this work. A new conotoxin, crSa, was isolated from the venom through Sephadex G-25 chromatography and HPLC, and then was identified by amino acid analysis, mass spectrometry, N-terminal sequence. The primary structure of crSa was CCKFQFLNFCCNE, it shared a conserved T-superfamily of conotoxins arrangement of cysteine residues （CC-CC）. Total chemical synthesis of the two possible disulfide linkages of cr5a was achieved, and the result of HPLC co-elution showed that cr5a was a tridecapeptide with a disulfide connectivity of Cys^1-Cys^10, Cys^2-Cys^11. So cr5a is a new member of the T-superfamily conotoxins.

关 键 词：芋螺毒素 T-超家族 独特芋螺 结构鉴定 

分 类 号：Q51[生物学—生物化学] O629[理学—有机化学]