羟基磷灰石与牛血清白蛋白相互作用的原位红外光谱研究  

In Situ ATR-FTIR Study on the Interaction of HA with Bovine Serum Albumin

作　　者：叶青[1] 胡仁[1] 林种玉[1] 林昌健[1] 

机构地区：[1]厦门大学化学系固体表面物理化学国家重点实验室,厦门361005

出　　处：《高等学校化学学报》2006年第8期1552-1554,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20273055;20021002;20423002);教育部博士学科点基金(批准号:20020384012)资助

摘　　要：In situ ATR-FTIR was used to study the interaction of bovine serum albumin（BSA） and hydroxyapatite（HA） in this work.Both on the electrochemically deposited HA and self assemblied titanate nanotubes（TNT） substrates,the adsorption of BSA increases with the increase of time,and its adsorption on HA surface is much larger than that on TNT surface,implying that HA has a good biocompatibility as a biomaterial.It is indicated that Ca2+ plays an essential role in the protein absorption on the HA surface,due to that BSA is negatively charged,and it is able to combine with cation locations in HA particles by electrostatic forces.It is found that,when reacting with BSA,the phosphate band of HA in solution shifts to higher wavenumbers,indicating that PO3-4 can also be an adsorption site for BSA.The spectroscopic study on the interaction between HA and BSA will be helpful for further understanding the nano hydroxyapatite bioproperties on molecular level.In situ ATR-fTIR was used to study the interaction of bovine serum albumin （BSA） and hydroxyapatite（HA） in this work. Both on the electrochemically deposited HA and self assemblied titanate nanotubes（TNT） substrates, the adsorption of BSA increases with the increase of time, and its adsorption on HA surface is much larger than that on TNT surface, implying that HA has a good biocompatibility as a biomaterial. It is indicated that Ca^2＋ plays an essential role in the protein absorption on the HA surface, due to that BSA is negatively charged, and it is able to combine with cation locations in HA particles by electrostatic forces. It is found that, when reacting with BSA, the phosphate band of HA in solution shifts to higher wavenumbers, indicating that PO4^3- can also be an adsorption site for BSA. The spectroscopic study on the interaction between HA and BSA will be helpful for further understanding the nano hydroxyapatite bioproperties on molecular level.

关 键 词：全反射红外光谱 羟基磷灰石 牛血清白蛋白 相互作用 

分 类 号：O646[理学—物理化学]