Caspase-3在肝癌组织中的表达及其与HBV感染的关系  

The expression significance of caspase-3 in the tissne of liver cancer and the relationship with HBV infection

作　　者：李玉中[1] 王朝晖[2] 王乃玉[3] 王丽丽[3] 邢文斌 

机构地区：[1]大连医科大学附属二院检验科,辽宁大连116027 [2]大连医科大学附属二院实验中心 [3]大连医科大学附属二院病理科 [4]大连市第六人民医院传染科

出　　处：《中国微生态学杂志》2006年第4期280-281,共2页Chinese Journal of Microecology

摘　　要：目的探讨天冬氨酸特异性半胱氨酸蛋白酶-3(Cysteinyl aspartate-specific proteinase-3,Caspase-3)和生存素(survivin)在原发性肝细胞肝癌(HCC)组织中的表达及其与乙肝病毒(HBV)感染的关系。方法用免疫组化SP法检测21例肝癌组织和10例癌旁组织Caspase-3和survivin的表达,并分析两者表达与HBV感染的关系。结果在肝癌和癌旁组织Caspase-3表达的阳性率分别为33.33%和66.67%(P<0.05),survivin在肝癌和癌旁组织表达的阳性率分别为85.71%和0(P<0.01)。Caspase-3在HBsAg阳性和阴性组表达的阳性率分别为27.78%和66.67%(P<0.05);survivin在HBsAg阳性和阴性表达的阳性率分别为88.89%和100%(P>0.05)。Caspase-3在高、中、低分化组表达的阳性率分别为66.67%、31.25%、33.33%,高分化组与中、低分化组比较差异有显著性(p<0.05);survivin在高、中、低分化组表达的阳性率分别为50%、87.5%、100%,高分化组与中、低分化组比较差异有显著性(p<0.05)。Caspase-3和survivin在HCC的表达呈显著负相关。结论HBV感染能使肝癌组织Caspase-3的表达降低,与survivin共同抑制肝细胞的凋亡,三者在HCC的发生及发展过程中起一定作用。Objective To investigate the effect of HBV infection to the caspase-3（Cysteinyl aspartate-specific proteinase-3） and survivin expression in primary liver cancer. Method Immunohistoehemistry was used to detect the expression of easpase-3 and survivin in 21 eases cancer tissue and 10 eases cancer surrounding tissue. Results In 21 eases cancer tissue and 10 eases cancer surrounding tissue, the positive expression ratio of easpase-3 were 33.33% and 70.00%, respectively （P〈0.05）; the positive expression ratio of survivin were 85.71% and 0, respeetively（P〈0.01）. In HBsAg positive and negative gruop, the positive expression ratio of easpase-3 were 27.78% and 100%, respectively （P〈0.05）. In high and low differentiation gruop, the positive expression ratio of caspase-3 were 66. 67% and 32. 29%, respectively （P〈0.05）; the positive expression ratio of survivin were 50.00% and 93.75%, respectively （P〉0.05）. The expression of easpase-3 and survivin in primary liver cancer was negatively correlated. Conclusion HBV could suppress the expression of easpase-3 in primary liver cancer, and this maybe the possible mechanism of primary liver cancer formation.

关 键 词：原发性肝细胞肝癌 CASPASE-3 SURVIVIN HBV 免疫组织化学 

分 类 号：R556.9[医药卫生—血液循环系统疾病]