适合棉铃虫细胞HzAm1生长的培养基筛选及低血清驯化  

Screen of Media for Insect Cells(HzAm1) Growth and Its Adaptation to Low Serum Culture

作　　者：张佑红[1] 王华林[2] 朱雄伟[1] 秦琴[1] 陈燕[1] 吕中[1] 马洁[1] 张菁[1] 

机构地区：[1]武汉工程大学省新型反应器与绿色化学工艺重点实验室,武汉430073 [2]中国科学院武汉病毒研究所,武汉430071

出　　处：《生物工程学报》2006年第4期686-688,共3页Chinese Journal of Biotechnology

基　　金：武汉市科技攻关项目(No.20052002044);湖北省重点实验室开放基金(No.RCT2004004)资助。~~

摘　　要：昆虫细胞-杆状病毒系统是昆虫杀虫剂生产和医用外源基因表达的有效工具。昆虫细胞的无血清或低血清培养是十分必要的。从三种商业化的培养基TC-100、GRACE和IPL-41中筛选出了最适合棉铃虫细胞HzAm1生长的基础培养基TC-100。以该培养基为基础,将血清用量从常用的10%降至1%,同时补加一定量的水解乳蛋白以及酵母提取物等,对棉铃虫细胞HzAm1进行驯化培养,效果良好。Insect cell-baculovirus system is a useful tool for both insecticidal virus production and the expression of medically useful foreign genes, Serum-free culture or low serum culture for insect cells is essential and significant. Media for the growth of insect cells HzAm1 from three kinds of commercial media TC-100, GRACE and IPL-41 with 10 % serum were investigated and screened. The result shows that medium TC-100 is the most suitable medium for the growth of cells HzAm1. Adaptation of insect cells HzAm1 to cultures in medium TC-100 with serum reduction from 10% to 1% was carried out as cultures were supplemented with lactalbumin hydrolysate and yeastolate etc. Growth of insect cells HzAm1 in medium TC-100 with serum 1% , lactalbumin hydrolysate and yeastolate was well.

关 键 词：棉铃虫细胞 无血清培养 细胞浓度 比生长速率 

分 类 号：S433[农业科学—农业昆虫与害虫防治]