中国大陆庚型肝炎病毒结构区与非结构区部分cDNA基因的序列分析  

SEQUENCES OF THE PARTIAL cDNA GENE OF HEPATITIS G VIRUS IN CHINESE

作　　者：程云[1] 郭仁锋[1] 李伯安[1] 罗清华[1] 曹阳[1] 张建宗[1] 貌盼勇[1] 马洪滨[1] 李静[1] 薛净[1] 

机构地区：[1]解放军第302医院

出　　处：《解放军医学杂志》1996年第4期257-259,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：作者在建立检测庚型肝炎病毒酶免疫和ＰＣＲ技术的基础上，将抗－ＨＧＶ阳性病人血清经逆转录─巢式ＰＣＲ分离部分ＨＧＶｃＤＮＡ片段，用与ＨＧＶ基因互补的特异寡酸引物进行双脱氧核苷酸链末端终止法──ＰＣＲ直接测序。结果表明，中国大陆ＨＧＶ－３０２Ⅰ株部分ｃＤＮＡ序列与Ｌｉｎｎｅｎ等报道的ＨＧＶ美国株ｃＤ－ＮＡ序列有极高的同源性。５’端非编码区、前１区、包膜Ｅ１区、ＮＳ３区和ＮＳ５区与美国株ｃＤＮＡ序列有高达９０％以上的同源性，编码相应氨基酸的同源性达９６％。表明ＨＧＶ感染虽呈全球分布，但在进化上属遗传高度保守。The partial cDNA genes of hepatitis G virus(HGV) were isolated from serum of a patient with chronic hepatitis C virus infection.Both of the positive anti HGV and the HGV RNA were detected by ELISA and PCR.The partial cDNA fragments from the 5′ non coding region、Pre E1、NS3 and NS5 regions were amplified.The total 655 nt of these cDNA fragments were sequenced and analysed.The results showed that the partial cDNA sequences from this patient was 92%～95% nucleotide identity and over 96% amino acid identity with the sequences of HGV cDNA in USA which was reported by Linnen et al .in 1995.It was shown that the HGV infection has a global distribution and the greatly homology of gene sequences of HGV in different countries will be beneficial to the development of universal vaccine and dignosis reagents.

关 键 词：庚型肝炎病毒 病毒 结构 CDNA 序列分析 

分 类 号：R512.63[医药卫生—内科学]