预先艾灸对随后不同阶段更年期大鼠促性激素释放激素、卵泡刺激素和黄体生成素的干预作用  

Effect of preventive moxibustion on Guanyuan point on gonadotroph in hormone-releasing hormone,follicle-stimulating hormone and luteinizing hormone of menopausal rats

作　　者：李晓泓[1] 解秸萍[1] 张露芬[1] 翟景慧[1] 周军[2] 周登方[1] 田阳春[1] 张慧[1] 郑玲[1] 何玉伟[1] 曲黎敏[3] 

机构地区：[1]北京中医药大学针灸学院,北京市100029 [2]安徽中医学院第一附属医院,安徽省合肥市230038 [3]北京中医药大学基础医学院,北京市100029

出　　处：《中国临床康复》2006年第31期99-101,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察逆灸关元穴对更年期大鼠促性激素释放激素、卵泡刺激素和黄体生成素等相关激素的影响,以及逆灸对更年期下丘脑-垂体-卵巢轴稳态维护的机制。方法:实验于2003-11/2004-06在北京中医药大学针灸学院针灸机理实验室完成。①选取鼠龄4个月的健康大鼠10只为年轻正常组,另将鼠龄10个月的大鼠70只数字表法随机分为2组,逆灸组(n=30),自然对照组(n=40),根据处死时的鼠龄,逆灸组又分12,14,16月龄3个时间点,自然对照组分10,12,14,16月龄4个时间点,每个时间点10只。②逆灸组于10月龄时采用艾炷直接灸大鼠关元穴,每次1壮,2次/周,灸后局部皮肤略潮红,共灸8周;其他2组不干预。③各组于相应时间点分批取材,采用放射免疫方法检测血浆卵泡刺激素、黄体生成素水平,下丘脑及血液促性激素释放激素水平。结果:大鼠80只全部进入结果分析。①血浆卵泡刺激素水平:自然对照组16月龄大鼠高于年轻正常组[(23.757±6.212),(10.565±0.817)U/L,P<0.05],逆灸组16月龄大鼠低于同月龄自然对照组[(17.952±2.231)U/L,P<0.05]。②血浆黄体生成素水平各组比较差异不显著(P>0.05)。③卵泡刺激素/黄体生成素比值:自然对照组16月龄大鼠大于年轻正常组(0.171±0.021,0.094±0.006,P<0.05),逆灸组16月龄大鼠小于同月龄自然对照组(0.125±0.007,P<0.05)。④下丘脑促性激素释放激素水平:自然对照组14,16月龄大鼠低于年轻正常组[(3.566±2.387),(3.032±0.602),(12.041±4.815)mg/L,P<0.05,0.01],逆灸组12,14,16月龄大鼠均高于同月龄自然对照组(P<0.05)。⑤血浆促性激素释放激素水平:自然对照组16月龄大鼠高于年轻正常组[(99.425±21.657),(23.816±6.058)ng/L,P<0.05],逆灸组12,16月龄大鼠显著高于同月龄自然对照组(P<0.05)。结论:更年期大鼠随月龄的增加,下丘脑-垂体-卵巢轴促性激素释放激素、卵泡刺激素和黄体生成素等相关激素逐渐紊乱,逆灸关元AIM：To analyze the mechanism of maintaining the order of hypothalamic-pituitary-ovarian （HPO） with preventive moxibustion by observing the effect on gonadotroph in hormone-releasing hormone （GnRH）, follicle-stimulating hormone （FSH）, luteinizing hormone （LH） of menopausal rats with preventive moxibustion on the Guanyuan point. METHODS：The experiment was conducted in the Laboratory of Acupuncture and Moxibustion College, Beijing University of Traditional Chinese Medicine between November 2003 and June 2004. ①Ten healthy rats of 4 months old were selected to be the normal young group, and another 70 rats of 10 months old were randomly divided into two groups： preventive moxibustion group （n=30） and natural control group （n=40）. The preventive moxibustion group was divided into 12-month, 14-month and 16-month groups according to the age of rats, and the natural control group was divided into 10-month, 12-month, 14-month and 16-month groups with 10 rats in each subgroup. ② In the preventive moxibustion group, moxibustion was applied to rat＇s Guanyuan point directly at the 10^th month with one zhuang every time and twice a week for continuous eight weeks, and the local skin slightly turned red after moxibustion. The other two groups received no intervention.③Materials were drawn from each group at corresponding time-point in batch. Radioimmunity was used to detect the levels of GnRH, FSH and LH of menopausal rats with preventive moxibustion. RESULTS：A total of 80 rats were involved in the analysis of results.① Level of FSH in serum： it was higher in the 16-month natural control group than in normal young group [（23.757±6.212）, （10.565±0.817） U/L,P 〈 0.05], and it was lower in 16-month preventive moxibustion group than the 16-month natural control group [（17.952±2.231）U/L,P 〈 0.05]② There were no significant differences in level of serum LH among each group （P 〈 0.05）. ③The ratio of FSH and LH： it was greater in the 16-month natural control

关 键 词：艾炷灸 更年期 大鼠 促性腺素释放激素 卵泡刺激素 黄体激素类 

分 类 号：R588[医药卫生—内分泌]