心脉龙干预后氧自由基代谢及脑、肝组织脂褐质含量的变化(英文)  

Effect of xinmailong on metabolism of oxygen free radicals and content of lipofuscin in brain and hepatic tissues

作　　者：吴建新[1] 钮荣祥[1] 黄秀群[1] 田昆仑[1] 董丛丽[1] 

机构地区：[1]大理学院基础医学院生理学与病理生理学教研室,云南省大理市671000

出　　处：《中国临床康复》2006年第31期188-190,共3页Chinese Journal of Clinical Rehabilitation

基　　金：云南省教委应用基础研究基金资助课题(20010716)~~

摘　　要：背景:心脉龙是从昆虫类中药美洲大蠊中发现并提纯制备的新型核苷类化合物。通过大量的实验研究表明,具有明显的心血管活性,可用于治疗急慢性心力衰竭及休克,明显改善微循环。目的:观察纯中药制剂心脉龙的抗衰老作用。设计:对照观察实验。单位:大理学院基础医学院生理学与病理生理学教研室。材料:实验于2002-07/2004-09在大理学院基础医学院机能学实验室完成。选用ICR小鼠40只,雌雄不拘,由昆明医学院云南省天然药物药理重点实验室提供。心脉龙注射液由云南腾冲制药厂生产[批号:94(ZL)03]。方法:40只ICR小鼠采用抽签法随机分为4组:青年对照组,衰老对照组,心脉龙大剂量组和心脉龙小剂量组,每组10只。青年对照组为3月龄小鼠,体质量(20±2)g,其余3组均饲养20月龄,体质量(40±3)g。从第20月开始,心脉龙小、大剂量组分别按6mg/kg、8mg/kg连续腹腔注射心脉龙30d,1次/d。青年和衰老对照组腹腔注射相同剂量的生理盐水。第31天,将实验小鼠断头取血、脑和肝脏,制成10%组织匀浆。按试剂盒说明书中的方法测定血清过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性,测定脑组织丙二醛含量及脑组织和肝脏脂褐质含量。主要观察指标:①血清过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性。②脑组织丙二醛含量及脑组织和肝脏脂褐质含量。结果:40只实验动物均进入结果分析,无脱失。①衰老对照组小鼠血清过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性明显低于青年对照组,而心脉龙两剂量组均能显著提高老年小鼠血清过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性,与衰老对照组比较,差异有显著性意义(P<0.05或P<0.01)。②衰老对照组小鼠脑组织丙二醛含量及脑组织和肝脏脂褐质含量明显高于青年对照组,而心脉龙大、小剂量�BACKGROUND： Chinese medicine xinmailong （XML） injection is a kind of new nucleoside compound which comes from American giant cockroach of insects. A lot of studies show that XML has obvious effect on cardiovascular activity which can treat acute or chronic congestive heart failure and shock and improve microcirculation. OBJECTIVE： To observe the effect of XML on anti-aging of mice. DESIGN： Controlled observational study. SETTING： Department of Physiology and Pathology, Basic Medical College of Dali College. MATERIALS： The experiment was carried out at the Functional Laboratory of Basic Medical College between July 2002 and September 2004. A total of 40 ICR mice of both sexes were provided by Kunming Medical College, Yunnan Naturally Occurring Drugs Key Laboratory. XML injection was provided by Yunnan Tengchong Pharmacy Factory [batch number： 94 （ZL） 03]. METHODS： A total of 40 ICR mice were randomly divided into 4 groups： young control group, aging control group, XML group at high dosage and XML group at low dosage with 10 in each group. All mice were fed routinely with standard feed. Mice in young control group aged 3 months and weighed （20＋2） g; meanwhile, mice in other groups aged 20 months and weighed （40~3） g. From the 20^th month, mice in XML groups were intraperitoneally injected with 6 mg/kg and 8 mg/kg, respectively, for 30 days with once every day. Mice in young and aging control groups were intraperitoneally injected with the same volume of saline.On the 31^st day, heads of mice were cut off to obtain blood which was maintained at hepalean anti-coagulated tube. Brains and livers were obtained rapidly for homogenate with 10% saline. Activities of catalase （CAT）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in serum, content of malondialdehyde. （MDA） in brain tissue and content of lipofuscin in cerebral and hepatic tissue were measured according to introduction of kits. MAIN OUTCOME MEASURES： ①Activities of CAT, SOD and GSHPx in seru

关 键 词：中草药 过氧化氢酶 超氧化物歧化酶 丙二醛 活性氧 

分 类 号：R339.38[医药卫生—人体生理学]