水飞蓟宾对小鼠肝脏炎症损伤和肿瘤坏死因子的产生及活性的影响  

EFFECT OF SILYMARIN ON MOUSE LIVER DAMAGE, PRODUCTION AND ACTIVITY OF TUMOR NECROSIS FACTOR

作　　者：张俊平[1,2] 胡振林[1,2] 冯增辉 林文[1,2] 余祥彬 钱定华[1,2] 

机构地区：[1]第二军医大学药学院中西药研究室 [2]北京四环药厂

出　　处：《药学学报》1996年第8期577-580,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：建立了小鼠肝损伤模型及体内外肿瘤坏死因子（ＴＮＦ）诱生的方法，研究水飞蓟宾（ＳＢ）对肝脏炎症损伤、ＴＮＦ产生及其生物活性的影响。结果表明∶ＳＢ在体内外均可显著抑制脂多糖（ＬＰＳ）诱导ＴＮＦ产生；在体外能抑制ＴＮＦ对肝细胞系ＧＳＧ７７０１和成纤维细胞系Ｌ９２９的细胞毒作用；在体内对ＬＰＳ诱导的痤疮丙酸杆菌致敏的小鼠肝脏炎症损伤有保护作用。提示ＳＢ的保肝作用机理与其抑制ＴＮＦ的产生和活性有关。Tumor necrosis factor (TNF) has been well characterized as a prominent mediator in the development of liver injury. Effects of silymarin(SB) on mouse liver damage, TNF production and activity were studied. Pretreatment with SB(25～50 mg\5kg -1 , ip, bid×3 d) before the lipopolysaccharides (LPS) injection markedly alleviated liver injury and diminished LPS induced TNF production in Propionibacterium acnes (PA) primed mice. SB(12.5～50 μg\5ml -1 ) significantly inhibited LPS induced TNF release from mouse peritoneal macrophage in a concentration dependent manner. SB(12.5～100 μg\5ml -1 ) was also shown to markedly reduce TNF cytotoxicity on human hepatic cell line GSG 7701 and mouse fibroblastic cell line L929 cells concentration dependently. These results suggest that inhibition of TNF production and its actions may be involved in the mechanism of protective action of SB on liver damage.

关 键 词：水飞蓟宾 肿瘤坏死因子 肝损伤 脂多糖 

分 类 号：R975.5[医药卫生—药品]