高效液相色谱荧光法测定Beagle犬血浆中酒石酸美托洛尔对映体浓度  

Determination of Metoprolol Tartrate Enantiomers in Beagle Dogs by HPLC Fluorescence Method

作　　者：刘雪英[1] 张巍巍[1] 苗青[1] 杨默媛[1] 侯建伟[1] 王庆伟[1] 张生勇[1] 

机构地区：[1]第四军医大学,西安市710032

出　　处：《中国药房》2006年第15期1130-1132,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(20372083)

摘　　要：目的：建立Beagle犬血浆中酒石酸美托洛尔对映体浓度的高效液相色谱荧光测定方法。方法：Beagle犬口服消旋体酒石酸美托洛尔100rag后取1．5h血样进样测定，手性色谱柱为Chiralcel OD—H，流动相为正己烷-异丙醇-二乙胺（65：35：0．1），荧光检测波长Ex和Em分别为267nm、290nm，柱温为25℃，流速为0．6ml／min，内标物为（S）-阿替洛尔。结果：酒石酸美托洛尔2种对映体检测浓度在10～2000ng／m1范围内线性关系良好，平均回收率为96．12％～116．9％（RSD〈7．6％）。结论：该分析方法选择性好、准确性高、重现性好，适于酒石酸美托洛尔对映体的药动学研究。OBJECTIVE： To establish HPLC method for the determination of plasma metoprolol tartrate enantiomers in Beagle dogs. METHODS： Plasma samples were taken from Beagle dogs at 1.5h after oral administration of metoprolol tartrate 100mg, Chiralcel OD H was taken as the chiral chromatographic column,the mobile phase consisted of N - hexane - dimethyl carbinol - diethylamine （65 ： 35 ： 0.1） with a flow rate at 0.6ml/min ,the fluorescence detection wavelength Ex was 267nm and Em was 290nm, the column temperature was 25℃ and the internal standard was （S） atenolol. RESULTS： Good linear relationship was achieved when the detection concentration of metoprolol enantiomers was within the range of 10--2 000ng/ml, the average recovery was 96.12% -- 116.9% （RSD〈 7.6% ） .CONCLUSIONS：The method is selective,accurate and reproducible, and suitable for the pharmacokinetics study of metoprolol tartrate enantiomers. KEY WORDS Metoprolol tartrate; Enantiomers; HPLC; Fluorometric method; Beagle dog

关 键 词：酒石酸美托洛尔 对映体 高效液相色谱 荧光法 BEAGLE犬 

分 类 号：R917[医药卫生—药物分析学] R972[医药卫生—药学]