MTT法测定卵巢癌患者单药与联合用药的体外敏感性  

Detection the Chemosensitivity of Patients with Ovarian Cancer to Anticancer Drugs Used Either Alone or in Combination by MTT Assay in Vitro

作　　者：曹丽芝[1] 任华益[1] 刘人树[1] 曾东向[1] 

机构地区：[1]湖南省肿瘤医院临床药学室,长沙市410006

出　　处：《中国药房》2006年第15期1155-1157,共3页China Pharmacy

摘　　要：目的:采用四甲基偶氮唑盐(MTT)法测定卵巢癌患者术后单药与联合用药体外敏感性,为临床个体化化疗方案的选择提供参考。方法:采用临床常用的20种化疗方案所用药物对18例卵巢癌患者的新鲜标本进行MTT法体外敏感性测定。结果:不同类型的卵巢癌及同一类型的不同个体对化疗药物的敏感性差异较大;联合用药较单一用药的平均抑制率高。结论:卵巢癌化疗敏感性个体差异大。MTT法是一种简便而快速的测定肿瘤体外药物敏感性的试验方法,可用于卵巢癌临床个体化化疗方案的选择。OBJECTIVE：To determine the sensitivity of patients with ovarian cancer to anticancer drugs used either alone or combination after operation in vitro using MTT assay for references in the selection of clinical individualized chemotherapy. METHODS：Chemosensitivity of 18 cases of ovarian cancer fresh specimen to 20 drug therapies commonly used in the clinic were detected in vitro by MTT assay.RESULTS：The sensitivity to anticancer agents varied greatly between different types of ovarian cancer and between different individuals who share the same type of ovarian cancer.The average inhibition ratio on ovarian cancer cells was higher in drugs that used in combination than that used alone.CONCLUSIONS： There was a great difference in sensitivity to anticancer agents between different patients with ovarian cancer.MTT assay is a simple and rapid method for detecting chemosensitivity of anticancer drugs in vitro,and it is conducive to the selection of clinical individualized chemotherapy for patients with ovarian cancer.

关 键 词：卵巢肿瘤 MTT法 药物敏感性 化疗方案 

分 类 号：R979.1[医药卫生—药品]