高效液相色谱法测定乳酸左氧氟沙星脂质体凝胶及霜剂中主药的含量  

Content Determination of Levofloxacin Lactate Liposomes Gel and Cream by HPLC

作　　者：肖桂珍 罗顺德[2] 张先洲[2] 刘刚[2] 周本宏[2] 

机构地区：[1]暨南大学珠海市人民医院,珠海市519000 [2]武汉大学药学院,武汉市430060

出　　处：《中国药房》2006年第15期1174-1175,共2页China Pharmacy

基　　金：广东珠海市科技计划项目(编号:200585)

摘　　要：目的：建立以高效液相色谱法测定乳酸左氧氟沙星脂质体凝胶及霜剂中主药含量的方法。方法：色谱柱为ZorbaxSBC18，流动相为乙腈-0．05mol／L枸橼酸溶液-1mol／L乙酸铵溶液（30：69．14：0．86），流速为0．80ml／min，检测波长为293nm，进样量为20μl，结果：乳酸左氧氟沙星检测浓度在5.0～60．0μg／ml范围内线性关系良好（r=0．9998，n=6），平均回收率脂质体凝胶、霜剂分剐为100．82％～101．10％（RSD=1．74％～2．88％）、100．84％～101．33％（RSD=1．81％～3．03％）。结论：该方法灵敏、稳定、重现性好，用于含量测定结果准确可靠。OBJECTIVE： To establish a HPLC method for the content determination of levofloxacin lactate liposome gel and cream .METHODS： Zorbax C18 column was adopted the mobile phase was acetonitrile - 0.05mol/L citric acid - 1mol/L ammonium acetate （30 ： 69.14： 0.86）, the flow rate was 0.80ml/min, sample size was 20/al, and the detection wavelength was 293 nm. RESULTS： The method was linear over the range of 5.0-60.0μg/ml（r = 0.9 998,n= 6） ,the average recoveries of liposome gel and cream were 100.82% -101.10% （RSD= 1.74%-2.88%） and 100.84% -101.33% （RSD = 1.81%- 3.03% ）, respectively. CONCLUSIONS： The established method is sensitive, stable, and highly reproducible, which produces an accurate and reliable result for the content determination of this preparation.

关 键 词：高效液相色谱法 乳酸左氧氟沙星 脂质体 凝胶 霜剂 含量测定 

分 类 号：R927.2[医药卫生—药学]