病毒性心肌炎患儿NK细胞、LAK细胞活性及T细胞亚群的变化  

Changes of NK cell and LAK cell activities and T-lymphocyte subsets in children with viral myocarditis

作　　者：钟在英 方超平[2] 张灵芳 

机构地区：[1]武警广西总队医院儿科,南宁530003 [2]上海长海医院实验诊断科,上海200433 [3]武警广西总队医院心内科,南宁530003

出　　处：《武警医学》2006年第7期491-492,共2页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨机体的免疫状态与小儿病毒性心肌炎(VMC)发病的相互关系,为临床治疗提供依据。方法对29例病毒性心肌炎患儿和15例健康体检儿采用单克隆抗体标记的间接ABC免疫组化法测定T细胞亚群,3H-TdR标记测定NK细胞、LAK细胞活性。结果VMC患儿急性期外周血NK细胞、LAK细胞的活性及T细胞亚群CD3、CD4/CD8比值明显低于对照组,CD8明显高于对照组(P<0.01)。结论VMC患儿急性期存在NK细胞、LAK细胞功能紊乱,T细胞亚群表达及比例失调,证实了免疫细胞功能紊乱与VMC发病有关。Objeetove To investigate the relationship between immune status and development of viral myocarditis （VMC）in children, there by providing the basis for clinical treatment of the disease. Methods Twenty - nine children with viral myocarditis and 15 heathy chil- dren were detected for NK cell and LAK cell activities using 3 H - TdR method , and T - lymphocyte subsets using ABC immunohistochemical method. Results NK cell and LAK cell activities and the level of CD3, CD4/CD8 ratio were significantly decreased and the level of CD8 significantly increased（ P 〈 0.01） in acute phase of VMC in children. Conclusion In acute phase of VMC in children, the inmmune funtions of NK cells and LAK cells are impaired, and the expression and distribution of T - lymphocyte subsets are abnormal, proving that dysfuntion of inmmunocytes play a role in pathogenesis of VMC.

关 键 词：病毒性心肌炎 T细胞亚群 NK细胞 LAK细胞 小儿 

分 类 号：R725.422.1[医药卫生—儿科] R730.51[医药卫生—临床医学]