CCD二极管阵列检测-流动注射分析尿中的肌酐  

THE KINETIC METHOD FOR CREATININE DETERMINATION BY FLOW INJECTION ANALYSIS WITH CCD-ARRAY DETECTOR.

作　　者：渠凌丽[1] 黎源倩[1] 谷素英[1] 

机构地区：[1]四川大学华西公共卫生学院卫生检测教研室,成都610041

出　　处：《现代预防医学》2006年第8期1429-1431,共3页Modern Preventive Medicine

摘　　要：目的:研究流动注射-CCD二极管阵列检测分光光度法测定尿中肌酐含量的方法。方法:基于经典的Jaffe反应,肌酐能与碱性苦味酸生成橙红色复合物,利用化学动力学反应,采用CCD二极管阵列检测装置快速测定尿中肌酐的含量。本实验探讨了最佳反应条件和流动注射分析参数。结果:本方法的线性范围为1μg/ml^33μg/ml,检出限为1μg/ml。样品的加标回收率在81.63%~119.7%之间,精密度为2.74%~3.96%。将本方法与临床自动生化仪的测定结果比较,经t检验(t=0.179,P>0.05),两种方法测定结果无统计学差异。结论:本方法采样频率为20次/h,简便快速、分析成本低,实现了尿中肌酐含量的批样分析,获得了满意的结果。Objective： To explore a rapid and sensitive method for creatinine determination by flow injection analysis with charge coupled device （CCD） diode array detector. Methods： The work is based on the Jaffe reaction between creatinine and alkaline picrate reagent forming a complex with salmon pink. To eliminate interference, the kinetic method was adopted. Results： The optimum conditions of the reaction and flow injection were optimized. A gcozl linearity was obtained in the range of 1μg/ml-33 μg/ml and the detection limit was 1 μg/ml. The recoveries of spiked samples were in the range of 81.63% - 119.7%, and the precision was between 2.74 % and 3.96 %. There is no significant difference in the determined results between autobiochemical analyser and the proposed method （ t = 0.179, P 〉 0.05）. Conclusion： Under the optimum condition, the sampling rate is 20 times per hour. The method was used for urine samples determination with satisfactory results.

关 键 词：肌酐 流动注射分析 二极管阵列检测 分光光度法 

分 类 号：R33[医药卫生—人体生理学]