广东肾虚型哮喘病ACE基因的遗传多态性  

Genetic polymorphism of angiotensin converting enzyme(ACE) gene in kidney-deficiency asthma from Guangdong population

作　　者：郭奕斌[1] 吕英 蔡浩武[1] 陈永恒 成云水 叶小帆[1] 

机构地区：[1]中山大学医学部基础医学院医学遗传室,广州510080 [2]天河区妇幼保健院中医科,广州510610

出　　处：《中国优生与遗传杂志》2006年第8期20-22,共3页Chinese Journal of Birth Health & Heredity

基　　金：广东省科技计划项目(2002C30408)

摘　　要：目的探讨血管紧张素转移酶(ACE)基因插入/缺失多态性与肾虚型哮喘病易感性的关系,为今后的连锁分析打下基础。方法用中医诊断指标对肾虚型哮喘患儿进行初诊,应用扩增片段长度多态性(Amp-FLP)方法检测52例哮喘患儿及其家系以及72例正常儿童的ACE基因型,然后用Hardy-W e inberg定律进行遗传平衡状态分析,用χ2检验进行统计学处理。结果两组儿童ACE基因型(II型、ID型、DD型)频率的分布差异无显著意义(P>0.05)。等位基因I频率为0.692,等位基因D频率为0.308,杂合率为34.6%;I、D的传递规律与理论上预计的完全符合。结论肾虚型哮喘病ACE基因也存在插入/缺失多态性,其中DD基因型与肾虚型哮喘的易感性有关,可能是儿童哮喘的危险因素。I、D在世代中的传递完全符合孟德尔遗传规律。Objective： The relationship between the insertion/deletion polymorphism of angiotensin converting enzyme （ACE） gene and the susceptibility of kidney - deficiency asthma in children was investigated. Methods： The kidney - deficiency asthma children were primarily diagnosed by the standard of Chinese medicine. ACE genotypes were determined by amplified fragment length polymorphism （ Amp - FLP） in 52 asthmatic children, 72 healthy controls. The genetic equilibrium state of this population was analyzed by Hardy - Weinberg law. The statistics was analyzed by using χ^2 test. Results ： The ACE genotype （ II, ID and DD） distribution was no significantly different between the two groups （P 〉 0. 05）. Conclusion： DD genotype of ACE gene may be related to susceptibility of children with kidney - deficiency asthma and may be a risk factor in development of kidney - deficiency asthma. The transmission of alleles was completely in agreement with the Mendelian genetic law.

关 键 词：血管紧张素转移酶 肾虚型 哮喘 中医 多态性 扩增片段长度多态性 

分 类 号：R725.6[医药卫生—儿科]