紫红獐牙菜高效液相色谱指纹图谱的研究  

Study on HPLC fingerprint of Swertia punicea

作　　者：张秀桥[1] 田峦鸢[1] 侯俊杰[1] 陈家春[1] 刘焱文[1] 

机构地区：[1]湖北中医学院,湖北武汉430061

出　　处：《中国医院药学杂志》2006年第8期922-924,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:30271590)

摘　　要：目的:建立紫红獐牙菜药材甲醇溶解部分的高效液相色谱(HPLC)指纹图谱。方法:HPLC色谱法,色谱柱为Kro-masil C18(250mm×4.6mm,5μL),流动相为甲醇-水(水中含0.05%磷酸)-乙腈,梯度洗脱,流速为0.8mL·min-1,检测波长为254nm,柱温为30℃。结果:初步建立紫红獐牙菜药材的HPLC指纹图谱,标定了13个共有特征峰,并利用对照品对4个特征峰进行了鉴定。结论:该方法简便、可靠,进一步完善了紫红獐牙菜药材的质量标准。OBJECTIVE To establish an HPLC fingerprint of the methanol extract from Swertia punicea. METHODS A column of Kromasil C18 （250 min× 46mm,5μL） was used. The mobile phase conststed of methanol-water（containing 0. 05% H3PO4）-ACN, Gradientelution mode was adopted. Flowrate was 0. 8mL·min^-1. Wavelength of detection 254nrn. Column temperture was 30℃. RESULTS The fingerprint of Swertia punicea was established was containing 13 common peaks 4 common peaks were identified using reference substance. CONCLUSION It is a simple and reliable meathod in controlling the quality of Swertia punicea.

关 键 词：紫红獐牙菜 高效液相色谱法 指纹图谱 

分 类 号：R978[医药卫生—药品]