口蹄疫病毒YN株VPl-2A基因的克隆及遗传变异分析  

Cloning and Hereditary Variation Analysis of the VP1-2A Gene of Food-and-Mouth Disease Virus

作　　者：任林柱[1] 王兴龙[2] 段小宇[1] 梅英武[1] 刘锴[1] 王学理[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]中国人民解放军军事医学科学院军事兽医研究所,长春130062

出　　处：《吉林农业大学学报》2006年第4期444-446,共3页Journal of Jilin Agricultural University

基　　金：军事医学科学院科技创新基金资助项目;吉林省科技厅科技发展计划项目(20050549)

摘　　要：以口蹄疫病毒YN株RNA为模板，用设计的特异引物RT—PCR扩增出大小为1055bp的基因片段，并对PCR产物克隆测序进行序列分析。结果表明：供试病毒的VP1-2A基因的核苷酸序列与参考毒株的同源性为77．65％-93．00％，对应的氨基酸序列同源性为87％-97％。One pair of primers were designed and synthesized to amplify the VP1-2A gene of FMDV. The predicted 1 055 bp fragment had been obtained by RT-PCR from the RNA of FMDV YN strain. The result of cloning and sequencing analysis showed that the homology of nucleotide sequence of the VP1-2A gene between the strain and the referenced sequence of type O foot-and-mouth disease is between 77.65% - 93.00% ,and the homology of amino acids is between 87% - 97%.

关 键 词：口蹄疫病毒 VP1-2A基因克隆 遗传变异 

分 类 号：S852.65[农业科学—基础兽医学]