鸡p15^(INK4B)基因的克隆表达及纯化  

Clone Expression and Purification of p15^(INK4B) Gene of Chicken

作　　者：艾永兴[1] 张玉静[1] 胡薇[2] 郝军元[1] 邹亚学[1] 张英波[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]吉林农业大学生物技术学院,长春130118

出　　处：《吉林农业大学学报》2006年第4期453-457,461,共6页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(30471284)

摘　　要：为研究鸡p15INK4B蛋白的功能及为制备此蛋白的单克隆抗体提供材料,应用引物搭桥法合成p15INK4B基因,应用基因工程的手段构建p15INK4B基因的原核表达载体,在E.coliRosetta(DE3)菌进行融合表达,并对表达产物进行亲和层析纯化。结果成功地构建了p15INK4B基因原核表达载体,经亲和层析制备了较高纯度的目的表达产物。To study p15^INK4B protein and prepare its monoclonal antibody, p15^INK4B gene was obtained by overlap-ligation PCR and cloned into expression vector. Then p15^INK4B gene was expressed in E. coli Rosetta（DE3） and p15^INK4B fusion protein was purified by aflqnity chromatography.The results indicated that the recombinant expression vector pET28a（ ＋ ）-p15 was successfully constructed and relatively pure target protein was obtained.

关 键 词：P15^INK4B 细胞周期 原核表达 亲和层析 鸡 

分 类 号：S831[农业科学—畜牧学]