喘息与非喘息型病毒性肺炎患儿淋巴细胞亚群的研究  

Lymphocyte subsets in children with asthmatic and non-asthmatic viral pneumonia

作　　者：马兰[1] 侯安存[1] 吕芳[1] 卢炎[1] 

机构地区：[1]首都医科大学附属北京友谊医院儿科,北京100050

出　　处：《中国实用儿科杂志》2006年第8期577-580,共4页Chinese Journal of Practical Pediatrics

摘　　要：目的了解喘息与非喘息型病毒性肺炎患儿淋巴细胞亚群的改变。方法对2002—2004在北京友谊医院就诊的急性期喘息型病毒性肺炎患儿37例及非喘息型病毒性肺炎患儿32例,采用流式细胞仪对其外周血淋巴细胞亚群进行检测。并以30例健康儿童作为对照组。结果喘息组与非喘息组病毒性肺炎患儿急性期外周血T辅助淋巴细胞亚群Th1细胞百分率分别为(12·61±7·19)%及(17·32±9·92)%,与对照组[(9·16±9·90)%]比较差异均有显著的统计学意义(P<0·001);喘息组与非喘息组患儿Th1/Th2比值均高于对照组(P<0·05,P<0·01)。此外与非喘息组比较,喘息组CD4+/CD8+较高(P<0·05),但其他各项指标差异均无统计学意义。结论病毒性肺炎时不论是否发生喘息,均呈现Th1应答优势。喘息型病毒性肺炎患儿除CD4+/CD8+较非喘息型病毒性肺炎高外,其他各项淋巴细胞亚群差异无明显统计学意义。Objective To study the lymphocytes subsets in children with viral asthmatic pneumonia. Methods The pa- tients who were treated from 2002 to 2004 in Beijing Friendship Hospital were divided into two groups： asthmatic group and non-asthmatic group. Fresh peripheral blood samples of patients in two groups were tested through the flow cytometry. Thirty healthy children were included as the control group. Results The result showed the percentage of Th1 cell was （ 12.61±7. 19） % and（ 17.32±9.92） % respectively in asthmatic group and non-asthmatic group,and there was significant difference from the control group[ P 〈 0. 001 ） ; the ratio of Th1/Th2 in asthmatic group and non-asthmatic group was higher than that in control group（ P 〈0. 05, P 〈0. 01 ）. The percentage of CD4＋ was higher in asthmatic group than that in control group（ P 〈0.01）. The ratio of CD4＋/CD8＋ was higher in asthmatic group than that in non-asthmatic group （ P 〈 0. 05 ）, but there was no other significant difference between the two groups. Conclusion No matter what it is asthmatic or non-asthmatic,the cell-medlated Th1 type immunity reaction is increased in patients with viral pneumonia. Except for a higher ratio of CD4＋/CD8＋ in asthmatic group there was no other significant difference between asthmatic group and non-asthmatic group.

关 键 词：喘息型病毒肺炎 淋巴细胞亚群 流式细胞仪 

分 类 号：R72[医药卫生—儿科]