APA微囊低温保存中降温方法的建立  

Hypothermia Methods Used in Low Temperature Preservation of Alginate-Polylysine-Alginate Microcapsules

作　　者：田磊[1] 潘静坤[1] 高宇红[1] 崔忻[1] 薛毅珑[1] 

机构地区：[1]解放军总医院老年医学研究所细胞生物学研究室,北京市100853

出　　处：《中国康复理论与实践》2006年第6期487-488,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家863计划资助(No.2002AA216141)

摘　　要：目的探讨海藻酸钙-多聚赖氨酸-海藻酸钙(APA)微囊低温保存的降温方法。方法用静电法制作APA微囊,分别以程控降温法、冰箱梯度法或直接液氮法降温,最终将APA微囊保存于液氮中。复温后,在显微镜下观察APA微囊的破损情况,并用荧光标记的葡聚糖检测APA微囊膜通透性的变化。结果程控降温组为微囊完好率(91.2±1.57)%、皱缩率(3.1±0.81)%、破损率(5.7±2.62)%;冰箱梯度组为微囊完好率(85.3±1.42)%、皱缩率(5.2±0.74)%、破损率(9.5±3.81)%;直接冻存组为微囊完好率(14.5±1.57)%、皱缩率(84.1±3.47)%、破损率(1.4±2.62)%。冻存复温后,各组完好的APA微囊膜通透性无改变。结论程控降温法可较好地低温保存APA微囊并维持其通透特性。Objective To explore the methods of low temperature preservation for alginate-polylysine-alginate （APA） microcapsules. Methods APA microcapsules were prepared with static electricity, and underwent hypothermal treatment respectively through methods of program control, gradient by icebox and put in liquid nitrogen directly, finally preserved in liquid nitrogen. The form and permeability of APA microcapsules were checked after rewarming. Results The rates of integrity, crenation and damage were （91.2±1.57）%, （3.1±0. 81）% and （5. 7±2.62）% in the program control group; （85. 3±1. 42）%, （5. 2±0. 74）% and （9. 5±3.81 ）% in the gradient by icebox group; （14.5±1.57） %, （84. 1 ± 3.47） % and （1.4 ± 2.62）% in the directly put in liquid nitrogen group. The membrane permeability of full APA microcapsules after frozen and reworming was not changed obviously. Conclusion The program control method can preferably preserve APA microcapsules at low temperature and keep them having normal form and permeability.

关 键 词：海藻酸钙-多聚赖氨酸-海藻酸钙(APA)微囊 低温保存 膜通透性 

分 类 号：Q813.1[生物学—生物工程]