磷对平邑甜茶根系质膜H^+-ATPase活性的调控  

Regulation of Phosphor on the Activity of Root Plasma Membrane H^+-ATPase in Malus Hupehensis(Pamp)Rehd.

作　　者：马方放[1] 杨洪强[1] 

机构地区：[1]山东农业大学园艺与工程技术学院,山东泰安271018

出　　处：《山东农业科学》2006年第4期17-19,共3页Shandong Agricultural Sciences

摘　　要：用含NaH2PO4浓度分别为0、0.2、0.4、0.6、0.8、1.0、1.2 mmol/L的营养液处理后,平邑甜茶根系质膜H+-ATPase的活性随着根区磷浓度的升高而升高,并在1.0 mmol/L处达到峰值;该酶的对硝基苯磷酸(PNPP)水解活性也明显升高,其Km值由3.48 mmol/L下降至0.97 mmol/L;同时结合抑制剂试验,钒酸钠对幼苗根系质膜H+-ATPase的抑制效应也得到了加强。After treated with the nutrient solution containing 0,0.2,0.4,0. 6,0.8, 1.0, 1.2 mmol/L NaH2PO4 respectively, the activity of root plasma membrane H^＋ -ATPase in Malus hupehensis （Pamp） Rehd. was obviously enhanced and reached the peak value at 1.0 mmol/L. PNPP hydrolysis activity was also promoted by phosphor,and its Km value decreased from 3.48 to 0. 97 mmol/L. And it was also found that 1.0 mmol/L NaH2PO4 could raise the inhibition of H^＋-ATPase activity by sodium vanadate. These results indicated that phosphor might regulate the activity of H^＋- ATPase by means of affecting the phosphatase domain of this enzyme.

关 键 词：磷 质膜H^+-ATPASE 对硝基苯磷酸(PNPP) 钒酸钠 

分 类 号：S571.101[农业科学—茶叶生产加工]