在副肿瘤性天疱疮患者中通过自身抗体识别包斑蛋白L亚区抗原表位  

Epitopes in the linker subdomain region of envoplakin recognized by autoantibodies in paraneoplastic pemphigus patients

作　　者：朱国兴 

机构地区：[1]Department of Dermatology, Peking University First Hospital, 8, Xishiku St., Beijing 100034, China

出　　处：《世界核心医学期刊文摘（皮肤病学分册）》2006年第6期14-15,共2页Digest of the World Core Medical JOurnals:Dermatology

摘　　要：Sera from paraneoplastic pemphigus (PNP) immunoprecipitate multiple antigens from human epidermal protein extract. In this study, we further characterized the autoantibodies in 12 PNP sera. Immunoblotting using recombinant linker subdomains of envoplakin, periplakin, desmoplakin, and bullous pemphigoid antigen I found that 11 of the 12 sera recognized linker subdomains of envoplakin and periplakin. We then synthesized 12 peptides covering the linker subdomain of envoplakin for ELISA. One of the peptides, peptide no. 8, was recognized by nine out of the 12 sera with a higher affinity. A method of ligandreceptor binding assay was designed and performed using this peptide labeled with fluorescence as the ligand. Peptide no. 8 bound to CD20+cells in Castlemans tumors from the patients whose sera were positive to this peptide by ELISA. Our data suggest that the linker subdomain of plakin proteins may be one of the major areas recognized by PNP autoantibodies, and epitopes in the linker subdomain of envoplakin recognized by PNP autoantibodies with a high affinity are dispersed in several areas and are variable among PNP patients. We also demonstrate that Blymphocyte clones specifically reacting to epidermal proteins exist in Castlemans tumors from PNP.Sera from paraneoplastic pemphigus （PNP） immunoprecipitate multiple antigens from human epidermal protein extract. In this study, we further characterized the autoantibodies in 12 PNP sera. Immunoblotting using recombinant linker subdomains of envoplakin, periplakin, desmoplakin, and bullous pemphigoid antigen I found that 11 of the 12 sera recognized linker subdomains of envoplakin and periplakin. We then synthesized 12 peptides covering the linker subdomain of envoplakin for ELISA. One of the peptides, peptide no. 8, was recognized by nine out of the 12 sera with a higher affinity. A method of ligand-receptor binding assay was designed and performed using this peptide labeled with fluorescence as the ligand. Peptide no. 8 bound to CD20^＋ cells in Castleman＇s tumors from the patients whose sera were positive to this peptide by ELISA. Our data suggest that the linker subdomain of plakin proteins may be one of the major areas recognized by PNP autoantibodies, and epitopes in the linker subdomain of envoplakin recognized by PNP autoantibodies with a high affinity are dispersed in several areas and are variable among PNP patients. We also demonstrate that B-lymphocyte clones specifically reacting to epidermal proteins exist in Castleman＇s tumors from PNP.

关 键 词：副肿瘤性天疱疮 蛋白提取物 自身抗体 抗原表位 Castleman肿瘤 患者 识别 大疱性类天疱疮 ELISA检测 受体结合分析法 

分 类 号：R758.66[医药卫生—皮肤病学与性病学] R737.33[医药卫生—临床医学]