扁平苔藓与人类疱疹病毒7型复制的关系及浆细胞样树突状细胞的浸润  

Lichen planus is associated with human herpesvirus type 7 replication and infiltration of plasmacytoid dendritic cells

作　　者：De Vries H.J.C. Van Marle J. Teunissen M.B.M. 罗素菊 

机构地区：[1]Department of Dermatology, Academic Medical Centre, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, Netherlands

出　　处：《世界核心医学期刊文摘（皮肤病学分册）》2006年第6期33-34,共2页Digest of the World Core Medical JOurnals:Dermatology

摘　　要：Background: Lichen planus (LP) is a common inflammatory skin disease of unknown aetiology. Viral causes have been suggested. Objectives: To find candidate viruses associated with LP.Methods: Lesional and nonlesional skin samples,peripheral blood mononuclear cells and serum were obtained from patients with LP. Ultrastructural, viral DNA, immunohistochemical and serological analyses were performed, and comparisons were made with psoriatic and normal skin. Results: Electron microscopy revealed typical 120-200-nm enveloped particles with a 100-nm nucleus resembling human herpesvirus (HHV) virions both in dermis and in epidermis of lesional LP tissue. HHV-7 DNA was found in 11 of 18 lesional LP samples, as opposed to only one of 11 nonlesional LP samples (P =0.06), two of 11 lesional psoriasis samples (P = 0.05) and none of four normal skin samples. No relation was found between LP skin and DNA of other known HHVs (HHV-1,-6 and 8). With immunohistochemistry, significantly more HHV-7+cells were found in lesional LP epidermis than in normal epidermis. Lesional LP dermis contained significantly more HHV-7+cells than nonlesional LP, psoriatic or normal dermis. Moreover, LP skin contained overwhelmingly and consistently more plasmacytoid dendritic cells (upregulated in virally induced conditions) than nonlesional LP samples. Conclusions: We conclude that HHV-7 replicates in LP lesions, but not in psoriasis, another inflammatory skin condition. HHV-7 is possibly involved in the pathogenesis of LP. These preliminary data make further research on this topic of interest.Background： Lichen planus （LP） is a common inflammatory skin disease of unknown aetiology. Viral causes have been suggested. Objectives： To find candidate viruses associated with LP. Methods： Lesional and nonlesional skin samples, peripheral blood mononuclear ceils and serum were obtained from patients with LP. Uhrastructural, viral DNA, immunohistochemical and serological analyses were performed, and comparisons were made with psoriatic and normal skin. Results： Electron microscopy revealed typical 120- 200-nm enveloped particles with a 100-nm nucleus resembling human herpesvirus （HHV） virions both in dermis and in epidermis of lesional LP tissue. HHV -7 DNA was found in 11 of 18 lesional LP samples, as opposed to only one of 11 nonlesional LP samples （P = 0.06）, two of 11 lesional psoriasis samples （P = 0.05） and none of four normal skin samples. No relation was found between LP skin and DNA of other known HHVs （HHV - 1, - 6 and 8） With immunohistochemistry, significantly more HHV -7 ＋ cells were found in lesional LP epidermis than in normal epidermis. Lesional LP dermis contained significantly more HHV -7 ＋ cells than nonlesional LP, psoriatic or normal dermis. Moreover, LP skin contained overwhelmingly and consistently more plasmacytoid dendritic cells （up nonlesional regulated in virally induced conditions） than LP samples. Conclusions： We conclude that HHV- 7 replicates in LP lesions, but not in psoriasis, another inflammatory skin condition. HHV- 7 is possibly involved in the pathogenesis of LP. These preliminary data make further research on this topic of interest.

关 键 词：浆细胞样树突状细胞 人类疱疹病毒7型 扁平苔藓 复制 炎症性皮肤病 银屑病皮损 免疫组化分析 HHV-7 外周血单核细胞 皮肤标本 

分 类 号：R512.91[医药卫生—内科学] R373.11[医药卫生—临床医学]