慢性前列腺炎患者前列腺液活性氧表达变化及意义  

Reactive oxygen species levels in expressed prostatic secretion in patients with chronic prostatitis

作　　者：张敏建[1] 陈康[1] 翟芳同[1] 于开源[1] 刘锦志[1] 谭育红[1] 史亚磊[1] 郑胜[1] 程宛钧[1] 

机构地区：[1]福建中医学院附属医院男科,福州350004

出　　处：《中华泌尿外科杂志》2006年第8期516-519,共4页Chinese Journal of Urology

基　　金：福建中医学院附属教育厅资助项目(JA02229)

摘　　要：目的探讨慢性前列腺炎(CP)患者前列腺液(EPS)中活性氧(ROS)表达变化与CP类型、EPS中WBC计数的相关关系。方法按照NIH分类标准,根据EPS常规检查,二杯法细菌培养,诊断CP患者87例,其中Ⅱ型10例、ⅢA型30例、ⅢB型30例、Ⅳ型17例,26例健康志愿者作正常对照组。显色法检测各组ROS值并进行相关分析。结果各组ROS数据均符合正态分布, P>0．1。其中Ⅱ型组ROS均值(233．1±56．1)U／ml,ⅢA型组(180．5±70．7)U／ml,ⅢB型组(156．8±38．9)U／ml,Ⅳ型组(124．9 4-34．1)U／ml,正常对照组(87．9±26．3)U／ml。CP患者EPS中ROS值明显高于对照组。Ⅱ型组、ⅢA型组、ⅢB型组、Ⅳ型组、正常对照组的ROS水平呈现从高到低的变化规律,其中Ⅱ型与Ⅲ型、ⅢA型与Ⅳ型之间差异有统计学意义(P<0．01),ⅢB型与Ⅳ型、Ⅳ型与正常对照组之间差异有统计学意义(P<0．05)。ⅢA型与ⅢB型组间差异无统计学意义(P> 0．05)。CP患者EPS中WBC计数与ROS水平呈正相关性(r=0．275,P=0．01),87例患者按EPS中WBC数分组:0—9个／HP组30例,ROS为(153．7±35．9)U／ml;10～19个／HP组16例,ROS为(153．3±44．8)U／ml;20～49个／HP组24例,ROS为(163．7±57．1)U／ml;≥50个／HP组17例,ROS为(205．3±929)U／ml。各组间ROS值差异有统计学意义(F=3．297,P<0．05)。卵磷脂小体含量与ROS水平呈负相关性(r=-0．31,P<0．01),87例患者按EPS中卵磷脂小体含量分组:(+)组22例, ROS值为(187．8±76．4)U／ml;(++)组32例,ROS值为(173．6±53．7)U／ml;(+++)组24例, ROS值为(160．0±49．7)U／ml;(++++)组9例,ROS值为(117．0±43．5)U／ml。各组间ROS值差异有统计学意义(F=3．363,P<0．05)。结论CP患者EPS中ROS表达增高,并参与前列腺炎的发生发展,可作为CP临床诊断和严重程度定量参考依据之一。Objective To investigate the levels of reactive oxygen species （ROS） in expressed prostatic secretion （EPS） in patients with chronic prostatitis （CP）,and to study the relationship between ROS and mechanism of CP. Methods According to NIH classification, ROS levels were measured by coloration in the EPS of 87 CP patients （ 10 cases of type Ⅱ , 30 of type ⅢA, 30 of type ⅢB and 17 of type Ⅳ ） and 26 normal individuals. Urine was cultured and EPS was studied according to 2-glass test. Results The ROS values were in normal distribution among the groups. The mean ± standard error of ROS levels was higher in 11 （233. 1 ±56. 1）U/ml, ⅢA （180.5 ±70.7）U/ml, ⅢB（156.8 ±38.9）U/ml and Ⅳ groups （ 124.9 ± 34.1 ） U/ml than that in controls （ 87.9 ± 26.3） U/ml,showing a tendency from high to low values. There were statistically significant differences between any 2 groups （ P 〈 0.05 ） , but no statistically significant difference between Ⅲ A CP/CPPS and Ⅲ B CP/CPPS groups （ P 〉 0.05 ）. There was a positive correlation between ROS level and WBC count in EPS （r =0. 275,P =0.01 ）. The 87 CP patients were divided into 4 groups by WBC count in EPS. The mean ± standard error of ROS levels was （ 153.7 ±35.9）U/ mlin0-9 WBC/HP group （n =30）;（153.3 ±44.8）U/ml in 10 - 19 WBC/HP group （n = 16）; （ 163.7 ± 57.1 ） U/ml in 20 - 49 WBC/HP group （ n = 24） ; （205.3 ± 92.9 ） U/ml in 〉 50 WBC/HP group （ n = 17 ）. There were statistically significant differences between the 4 groups （ F = 3. 297, P 〈 0.05 ）. There was a negative correlation between ROS levels and lecithin in EPS（ r = -0. 31 ,P 〈 0.01 ）. The 87 CP patients were divided into 4 groups by lecithin contents. The mean ± standard error of ROS levels was （ 187.8 ± 76. 4） U/ml in （＋） group （n=22）;（173.6±53.7）U/mlin （ ＋ ＋） group （n=32）;（160.0±49.7）U/ ml in（ ＋ ＋ ＋ ）group （n =24） ;（117.0 ±43.5） U/ml in （ ＋ ＋ �

关 键 词：前列腺炎 活性氧 

分 类 号：R697.3[医药卫生—泌尿科学]