玉米幼胚组织培养及其转化的研究  

Study on Tissue Culture and Transformation of Maize

作　　者：赵云云[1] 周小梅[2] 王国英[3] 

机构地区：[1]首都师范大学生物系,北京100037 [2]山西大学生命科学与技术学院,太原030006 [3]中国农业大学农业生物技术国家实验室,北京100094

出　　处：《山西大学学报（自然科学版）》2006年第3期308-312,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：北京市教育委员会科技发展计划项目(01KJ-112)

摘　　要：对6个玉米自交系和15个杂交组合的幼胚在不同培养基中进行了愈伤组织诱导、继代、分化培养,所试材料都能诱导出状态良好的愈伤组织,并在继代培养中生长正常.使用D icam ba和A gNO3能促进和改善愈伤组织的诱导数量和质量,二者对愈伤组织的良好状态和分化能力的保持具有协同效应.在分化培养基中提高蔗糖的浓度和附加细胞分裂素有利于胚状体的形成和苗的分化.杂交组合的愈伤组织生长状态遗传了双亲的特点,正反交试验表明,其特征更接近母本.对自交系Z3和Z31进行基因枪转化,PCR及PCR-Sou thern检测证实外源基因的导入.Immature embryos of 6 inbred and 15-hybrid maize （Zea mays） were cultured on modified D or N6 medium. Callus was induced from all the genotypes tested and grow normally. The supplement of silver nitrate and Dicamba in media were found to improve both the quality and the quantity of the embryogenic calli. A synergistic effect between AgNO3 and Dicamba was observed for callus initiation and maintenance. High concentration of sucrose and cytokinin show to be most favorable for the formation of the embryogenic callus and shoot differentiate. The result of hybrid combination indicated that the capacity to form regenerable callus cultures in maize had a heritable basis. The study for 4 reciprocal crosses showed that there were probably some maternal effects on maize callus cultures. The lines of Z3 and Z31 were used for transformation by bombardment. PCR and PCR-Southern hybridization demonstrated that the extrogenous gene had integrated into the maize genome.

关 键 词：玉米 组织培养 转化 

分 类 号：Q813[生物学—生物工程]