维甲酸脂质体的制备及评价  

Preparation and characteristics of liposomal retinoic acid

作　　者：蔡明志 黄复生[2] 王昆[2] 

机构地区：[1]解放军第一五四医院药剂科,信阳464000 [2]第三军医大学病原生物学教研室,重庆400038

出　　处：《中国新药杂志》2006年第14期1181-1183,共3页Chinese Journal of New Drugs

基　　金：中关村科技生物医药创新研究基金(2000-2)

摘　　要：目的:制备维甲酸脂质体并对其包封率、粒径、药物含量等进行考察。方法:采用逆向蒸发法制备维甲酸脂质体;高效液相色谱法测定药物含量和包封率;激光散射法和透射电镜测定和观察脂质体粒径和形态。结果:磷脂和胆固醇总的质量浓度在40～50mg·mL^(-1),磷脂与胆固醇的质量比在3:1～4:1时,得到包封率超过90%的维甲酸脂质体;粒径700～800nm,多分散系数(PI)＜0．3,电镜下观察为大单层脂质体。结论:维甲酸脂质体包封率较高,粒径大小均匀,含量符合要求。Objective：To optillice and characterize the liposomal retinoic acid.Mthods： The liposomal retinoic acid was prepared by reverse evaporation followed by microfludic technology;the concentration and encapsulation efficiency of retinoic acid in the liposomes were determined by high performance liquid chromatography;the particle shape and size and disiribution of liposomal retinoic acid were assessed by transmission electrical microscope and photon correlation spectroscopy.Results：The total concentration of phospholipids and cholesterol was 40-50mg·mL^-1（3%-4% of total weight）.At the mass ratio of phospholipids and cholesterol 3：1-4：1,the encapsulation efficiency of liposomal retinoic acid reached around 90%.The average particle size of liposomal retinoic acid was at 700-800nm.The retinoic acid was mostly in large unilamellar vesicles with ploydispersity index（PI） below 0.3.Conclusion：The homogeneous liposomal retinoic acid was achievable.

关 键 词：脂质体 维甲酸 包封率 

分 类 号：R977.2[医药卫生—药品] R944.9[医药卫生—药学]