高效液相色谱法测定替米沙坦血药浓度  

Determination of telmisartan by HPLC

作　　者：孙黎[1] 沈金芳[1] 陶达人[1] 祝德秋[1] 

机构地区：[1]上海第二医科大学附属仁济医院临床药学研究室,上海200001

出　　处：《中国新药杂志》2006年第14期1210-1212,共3页Chinese Journal of New Drugs

摘　　要：目的:建立测定人血浆中替米沙坦浓度的方法。方法:血浆样品用甲醇提取及沉淀蛋白。采用反相高效液相色谱-荧光法进行分离、测定。色谱柱采用Diamonsil^(TM)C_(18)(200mm×4．6mm,5μm),流动相为水-乙腈-三乙胺(73:27:0．1),柱温25℃,流速为1．0mL·min^(-1),荧光激发波长为305nm,发射波长为380nm。结果:线性范围为1～200ng·mL^(-1)(r=0．9999),最低检测浓度为1．0ng·mL^(-1),高、中、低3种浓度的方法回收率为97%～103%,提取回收率均大于93%。日内及日间精密度均＜7%。结论:本方法简单、快速,灵敏度和准确度较高,能满足替米沙坦临床药动学研究的需要。Objective： To establish a RP-HPLC method to determine the concentration of telmisartan in human plasma. Methods： Plasma samples purified were analyzed by a HPLC consisted of a Diamonsil^TM C18（200 mm×4.6 mm,5μm） column with a maintenance temperature at 25℃, an eluate of water-acetonitrile-triethylamine （73： 27： 0. 1, v/v/v） with a flow rate of 1.0 mL·min^-1, and detection with a fluorescence detector set at an excitation wavelength of 305 nm and emission wavelength of 380 rim. Results： A linearity of telmisartan concentration curve was obtained in a range of 1.0～200 ng·mL^-1 （r =0. 999 9）. The detection limit was 1.0 ng·mL^-1 , and the recovery was from 97% to 103%. The extraction recovery of telmisartan from plasma concentration was more than 93%. Both RSD within day and between days were less than 7% （n =5）. Conclusion： The stable and reliable RP-HPL is applicable to assess the pharmacokinetic profile of telmisartan.

关 键 词：替米沙坦 高效液相色谱法 荧光法 血药浓度 

分 类 号：R917.1[医药卫生—药物分析学] R972.4[医药卫生—药学]