单核细胞趋化蛋白-1及其受体在阴道念珠菌病小鼠模型中的表达  

Expression of MCP-1 and its receptor CCR2 in a murine model of experimental vaginal candidiasis

作　　者：严小枫[1] 涂亚庭[1] 林能兴[1] 黄长征[1] 刘志香[1] 邬炎卿[1] 张德美[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉430022

出　　处：《中华皮肤科杂志》2006年第8期466-469,共4页Chinese Journal of Dermatology

摘　　要：目的探讨单核细胞趋化蛋白-1(MCP-1)及其受体CC趋化因子受体2(CC chemokine receptor 2．CCR2)在小鼠阴道念珠菌病发病中的作用。方法建立阴道念珠菌病小鼠模型．以RT-PCR及ELISA方法检测小鼠阴道及腰淋巴结中MCP-1及CCR2的表达。结果与正常小鼠相比,雌激素对MCP-1及CCR2的表达无明显影响。所有感染组小鼠阴道组织MCP-1的mRNA和蛋白水平均明显升高,其CCR2 mRNA水平亦相应升高；与之相反,所有感染组小鼠的引流腰淋巴结MCP-1的浓度与基础水平相比无明显改变,而雌激素处理感染组小鼠CCR2在接种后7～21d明显升高。结论MCP-1及其受体CCR2可能在局部刚道黏膜白念珠菌感染中起一定的作用。Objective To investigate the role of monocyte chemoattractant protein-1 （ MCP-1 ） and its receptor CC chemokine receptor 2 （ CCR2 ） in vaginal infection with Candida albicans by detecting their expression in a murine model with experimental vaginal candidiasis. Methods A murine model of vaginal candidiasis was established, Mice infected with Candida albicans, and the non-infected control animals, were treated with estrogen. The expression of MCP-1 and CCR2 in the murine vaginal tissue and draining lumbar lymph nodes was assessed by RT-PCR and ELISA. Results Estrogen had no significant effect on the expression of MCP-1 or CCR2. Compared with the basal level of control mice, the protein and mRNA expression of MCP-1 as well as the CCR2 mRNA expression were significantly increased in the vaginal tissue of infected mice, while the protein and mRNA expression of MCP-1 in the draining lumbar lymph nodes of infected mice remained unchanged. A significant increase in the mRNA expression of CCR2 was observed in estrogen- treated infected mice at day 7, 14 and 21 after the inoculation. Conclusion MCP-1 and its receptor CCR2 may play a role in the local vaginal mucosal response to C. albicans.

关 键 词：念珠菌病 外阴阴道 念珠菌 白色 单核细胞化学吸引蛋白质1 受体 趋化因子 

分 类 号：R711.3[医药卫生—妇产科学]