MMP-2表达与大鼠动脉粥样硬化和血管钙化的关系及辛伐他汀对其影响  

Relation between expression of MMP-2 and artery calcification in rats,and the effect of Simvastatin

作　　者：卢维晟[1] 王一尘[1] 

机构地区：[1]上海第二医科大学附属新华医院老年科,上海200092

出　　处：《心脏杂志》2006年第4期376-378,共3页Chinese Heart Journal

摘　　要：目的 观察基质金属蛋白酶-2（MMP-2）随动脉粥样硬化和血管钙化病理变化过程的量效变化，并探讨辛伐他汀对其影响。方法 健康雄性SD大鼠44只，体质量150～200g。随机分为5组：①对照组；②VitD3组；③高脂组；④高脂高钙组；⑤辛伐他汀组。应用免疫组化技术，对动脉粥样硬化大鼠主动脉MMP-2的表达进行测定。结果 高脂高钙组的MMP-2表达高于高脂组（P〈0．05）。辛伐他汀组的MMP-2表达低于高脂高钙组（P〈0．05）。辛伐他汀能抑制主动脉MMP-2的表达，并且随着MMP-2表达程度的降低，主动脉钙化程度也有所改善。结论 MMP-2表达与钙代谢及血管钙化密切相关。辛伐他汀减轻主动脉钙化的作用可能是通过影响MMP-2表达水平而实现的。AIM To observe the changes of MMP-2 level during atherosclerosis and artery calcification, and to investigate the mechanism of simvastatin affecting the vessel calcium metabolism. METHODS Forty-four male SD white rats, with body weight of 150 -200 g, were divided into five groups at random：① baseline group（ n = 8 ） ： normal diet ; ② VitD3 group（ n = 9 ） ： normal diet ＋ VitD3 ; ③ high-cholesterol diet group（ n = 9 ） ：high cholesterol diet ; ④ high-cholesterol-calcium diet group （ n = 9 ） .. high cholesterol and high calcium diet; ⑤Simvastatin group（ n = 9 ） ：high cholesterol and high calcium diet, with simvastatin 10 mg/（ kg ·d） added. The content of MMP-2 in artery walls was determined by immunohistochemistry method. RESULTS The high-cholesterol-calcium diet group, compared with high-cholesterol diet group, had higher expression of MMP-2 （P 〈 0.05 ）. While compared with high-cholesterol-calcium diet group, MMP-2 expression of the simvastatin group was lower （P 〈 0.05）. CONCLUSION MMP-2 expression is correlated with calcium metabolism and artery calcification. Simvastatin can restrain the expression of MMP-2 in artery and therefore alleviate the artery calcification.

关 键 词：动脉硬化 血管钙化 辛伐他汀 基质金属蛋白酶-2 

分 类 号：Q344[生物学—遗传学] R972[医药卫生—药品]