雪旺细胞和神经干细胞共移植治疗大鼠帕金森病的实验研究  

Co-transplantation of Schwann cells and neural stem cells in PD rats

作　　者：江澄川[1] 夏鹰[1] 汪洋[2] 丁忠良[2] 徐斌[1] 冯林音[3] 

机构地区：[1]复旦大学华山医院神经外科,上海200040 [2]复旦大学解剖与组胚教研室,上海200032 [3]中国科学院神经所,上海200031

出　　处：《中华神经医学杂志》2006年第8期785-788,共4页Chinese Journal of Neuromedicine

摘　　要：目的研究雪旺细胞(SCs)和神经干细胞(NSCs)共移植治疗大鼠帕金森病(PD)的疗效。方法18只PD模型的SD大鼠随机平均分成3组：对照组、NSCs组及共移植组。PBS、NSCs及SCs加NSCs分别植入PD大鼠右侧纹状体内。NSCs来源于孕14～16 d胎鼠中脑腹侧的脑组织,SCs来源于1～3 d SD新生鼠的坐骨神经。结果移植10周后NSCs组及共移植组中纹状体区都出现TH染色阳性的神经元,与NSCs组相比,共移植组中的神经元体积及细胞核均较大,细胞数量多(P＜0．05)。与对照组相比,NSCs组及共移植组中大鼠的行为学均有明显的好转(P＜0．01),但NSCs组和共移植组相比,行为学的改变无明显差异(P＞0．05)。结论SCs和NSCs共移植能有效治疗PD模型大鼠。Objective To explore the curative effect of co-transplantation of Schwann cells （SCs） and neural stem cells （NSCs） in Parkinson＇ s disease （PD） rats. Methods Eighteen hemiparkinsonian SD rats with right substantia nigra lesioned were selected by estimation of rotational behavior in response to apomorphine and randomly assigned to 3 groups： control group, NSCs group and co-graft group, with 6 rats in each group. PBS, NSCs, SCs plus NSCs were transplanted into the right 6-OHDA lesioned neostriatum of the PD rat respectively. The mesencephalic NSCs were obtained from the brain of SD rats at embryonic day 13-16 and SCs came from the sciatic nerves of neonatal rats at day 1-3. Results TH immunoreactive neurons emerged in the striatum of both NSCs group and co-graft group 10 weeks after transplantation. The volumes and nucleus of neurons in co-graft group were bigger than the ones of NSCs group and so do the number of the DA neurons （P〈0.05）. Compared with control group, the percent of apomorphine-induced rotation in both NSCs group and co-graft group was decreased significantly（P〈0.01）, but no difference was found between them （P〉0.05）. Conclusion Co-transplantation of SCs and NSCs is effective in treating PD rats. Not only dopaminergic neurons differentiated from NSCs functioned in the unilateral 6-OHDA lesioned neostriatum, but also SCs improved the survival of NSCs by secreting many neurotrophic factors.

关 键 词：雪旺细胞 神经干细胞 帕金森病 移植 

分 类 号：R651[医药卫生—外科学]