聚合物基因载体的设计与优化  

Design and optimization of polymers for gene delivery

作　　者：要旸[1] 侯森[1] 冯喜增[1] 

机构地区：[1]南开大学生命科学学院生物活性材料教育部重点实验室,天津市300071

出　　处：《中国临床康复》2006年第33期125-129,共5页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助(90403140)~~

摘　　要：目的:随着基因治疗研究的不断深入,选择更安全和更有效的基因转移载体已成为基因治疗成功的关键。为此本文就聚合物基因载体的设计与优化进行综述。资料来源:应用计算机检索PUBMED1990-01/2005-12期间的相关文章,检索词为“genetherapy,non-viralvectors,cationicpolymer/DNAcomplex”,并限定文章语言种类为English。同时计算机检索万方中国全文期刊数据库1990-01/2005-12期间的相关文章,检索词为“基因治疗,非病毒载体,阳离子聚合物/基因复合物”,并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与聚合物基因载体的设计及优化研究相关。排除标准:重复研究或Meta分析类文章。资料提炼:共收集到60篇相关文献,28篇文献符合纳入标准,排除的32篇文献为内容陈旧或重复。符合纳入标准的28篇文献中,26篇涉及基因转移体系,2篇涉及Polyplex转染过程中的问题以及聚合物载体的优化。资料综合:本文首先简要介绍了两类基因转移体系:病毒载体转移体系和非病毒转移体系,以及非病毒转移体系的分类。然后详细介绍了聚合物基因载体的发展现状,列举了几类近年设计的新型阳离子聚合物载体,指出其优点和不足、结构与功能的关系,以及优化的方法。最后介绍了以阳离子聚合物作为基因载体进行细胞转染的机制,并探讨了如何对聚合物基因载体进行优化。结论:在非病毒转移体系中,聚合物基因载体由于其设计灵活及具有很好的生物相容性等优点而在人类基因治疗中具有很大的潜力。然而低转染效率限制了其在临床上的应用。随着对聚合物基因转移机制的进一步理解,聚合物基因转移体系将成为人类基因治疗的有力工具。OBJECTIVE： As the concept of gene therapy expanded, the choice for a safer and more efficient genetic transfer delivery becomes the key to successful gene therapy. In this paper, we summarize the design and optimization of polymers for gene delivery. DATA SOURCES： Using the key words of ＂gene therapy, non-viral vectors, cationic polymer/DNA complex＂, we searched the PUBMED database for relevant articles published in English between January 1990 and December 2005. Meanwhile, the online search of Chinese Journal Full-text Database was undertaken for relevant Chinese articles between January 1990 and December 2005 with the same key words in Chinese. STUDY SELECTION： All articles were selected firstly, and the references of each article were checked in detail. Inclusive criteria： Articles relevant to the design and optimization of polymers for gene delivery. Exclusive criteria： Duplicated articles or Meta analysis. DATA EXTRACTION： Among 60 selected articles, 28 one were included, 32 duplicated or out of date ones were excluded. In the 28 inclusive articles, 26 were relevant to the gene delivery systems, 2 were relevant to problems in gene transfection and optimization of polymers for gene delivery. DATA SYNTHESIS： Firstly, two categories of gene-delivery systems： viral and non-viral gene delivery system, and classification of non-viral gene delivery system were briefly introduced. Secondly, the development of polymers for gene delivery was expounded, and the advantages and disadvantages, the correlation between structures and functions, and their optimization strategies of several recently designed cationic polymers were described. Finally, the cell transfection mechanisms were presented with cationic polymers as the gene delivery vectors and the optimization strategies was discussed with consideration of the mechanisms. CONCLUSION： Cationic polymers, within the non-viral gene delivery system, have great potential for human gene therapy because of their flexibilityof design, good bioc

关 键 词：基因疗法 聚合物 遗传载体 基因转移技术 综述文献 

分 类 号：R318[医药卫生—生物医学工程]