许旺细胞源神经营养因子对脊神经根性撕脱所致神经元死亡的干预(英文)  

Protection of motoneurons from spinal root avulsion induced cell death by Schwann cell derived neurotrophic factor

作　　者：刘黎军[1] 朱家恺[2] 肖建德[1] 王大平[1] 杨雷[1] 

机构地区：[1]深圳市第二人民医院骨三科,广东省深圳市518035 [2]中山大学附属第一医院显微外科,广东省广州市510080

出　　处：《中国临床康复》2006年第33期168-170,F0003,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助(39470700)~~

摘　　要：背景:许旺细胞源神经营养因子是从许旺细胞胞浆中分离纯化出的一种相对分子质量为58000的神经营养物质,具有明显的神经营养活性,能对抗一氧化氮神经毒性物质。目的:建立根性撕脱伤动物模型,观察许旺细胞源神经营养因子对脊神经根性撕脱所致脊髓前角运动神经元死亡的保护作用。设计:重复观察测量。单位:深圳市第二人民医院骨三科,中山大学附属第一医院显微外科。材料:实验于2003-03/05在中山大学医学院实验动物中心完成。选用清洁级3～4月龄SD大鼠20只,随机分为许旺细胞源神经营养因子组、生理盐水对照组,各10只。两组动物均以左侧为正常侧,右侧为损伤侧。方法:①两组大鼠均建立颈6,7神经根性撕脱伤动物模型。②许旺细胞源神经营养因子组将一小块预浸有质量浓度为1g/L的许旺细胞源神经营养因子40μL的明胶海绵覆盖于损伤侧硬膜囊表面,生理盐水对照组将一小块预浸有等量生理盐水的明胶海绵覆盖于损伤侧硬膜囊表面。③明胶海绵表面置硅胶管,硅胶管一端与明胶海绵缝扎,另一端用凡士林封闭固定于皮下,关闭切口后伤口局部肌肉注射青霉素预防感染。术后两组动物分别通过硅胶管定期注射许旺细胞源神经营养因子或生理盐水20μL,1次/周,3周后取材。④切取颈6,7脊髓节段,观察损伤侧脊髓前角运动神经元的存活率和形态学变化以及一氧化氮合酶的表达。主要观察指标:①脊髓运动神经元存活情况和形态学变化。②脊髓运动神经元一氧化氮合酶表达的变化。结果:实验选用20只大鼠,全部进入结果分析。①脊髓运动神经元存活情况和形态学变化:颈6,7神经根性撕脱术后3周,生理盐水对照组损伤侧68.6%的运动神经元死亡,存活率31.4%,明显低于正常侧(P<0.01),且存活的神经元胞体严重萎缩;许旺细胞源神经营养因子组损伤侧脊髓运动神经元的�BACKGROUND： Schwann cell derived neurotrophic factor, which is isolated and purified from the kytoplasm of Schwann cell with the relative molecular mass of 58000, is a kind of neurotrophic substance possessing obvious neurotrophic activity. It can be against neurovirulent substance of nitrogen monoxidum. OBJECTIVE ：To create root avulsion animal models and observe the protective effects of Schwann cell derived neurotrophic factor （SDNF） on motoneurons of spinal anterior horn from spinal root avulsion induced cell death. DESIGN： Repeated observation and measure. SETTING： Third Department of Orthopaedics, Second People＇s Hospital of Shenzhen ; Department of Micro-surgery , First Hospital Affiliated to Sun Yat-sen University. MATERIALS： This experiment was conducted at the Experimental Animal Center of Medical College of Sun Yat-sen University from March to May 2003. Twenty Sprague-Dawley rats with the age of 3-4 months, of clean degree, were selected and divided randomly into experimental group of Schwann cell derived neurotrophic factor and control group of normal saline with 10 rats in each group. The right side was injured, and the left side was intact served as normal control side. METHODS ： ①A rat model of C6.7 spinal root avulsion induced motoneuron degeneration was established. ② A small piece of gelfoam presoaked in 40 μL SDNF solutions （1 g/L） was placed in contact with the injured spinal cord in the animals of the experimental group, Normal saline was added as the stone way as above in the animals of the control group, ③ A silica pipe was put on the surface of gleform, one end of the silica was sutured to the glefoam , and the other end was fixed subcutaneously with vaselinum, Local intramuscular injection of penicillinum was performed on the wound following closing the incision. All rats received an injection （20 μL） of either SDNF or normal saline solution at the lesion site through the silica pipe sutured to the glefoam once a week after the surgery, All the

关 键 词：许旺氏细胞/细胞学 运动神经元 一氧化氮 一氧化氮合酶 

分 类 号：R318[医药卫生—生物医学工程]