烫伤大鼠创面脓毒症发生后肝组织中脂代谢相关基因表达水平的变化  

Changes in the expression of rat hepatic lipid metabolism related genes in response to burn wound sepsis as assessed by DNA arrays

作　　者：郝岱峰[1] 柴家科[1] 吴焱秋[1] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所,北京100037

出　　处：《中华烧伤杂志》2006年第4期250-253,共4页Chinese Journal of Burns

基　　金：国家自然科学基金资助项目(39970716)

摘　　要：目的检测烫伤大鼠创面脓毒症发生后肝脏组织中脂代谢相关基因表达水平的变化,并分析其意义。方法将60只背部30％TBSAⅢ度烫伤大鼠随机分为创面脓毒症组和对照组,每组30只。创面脓毒症组大鼠创面涂布铜绿假单胞菌菌液,并对相应指标进行检测以确定该组大鼠是否符合创面脓毒症诊断标准。对照组除创面不涂菌液外,其余操作及检测同创面脓毒症组。两组大鼠于伤后96 h断颈处死,通过基因芯片杂交方法检测两组肝组织中表达水平有显著差异的基因,并按其功能筛选出与脂代谢相关的基因。结果两组大鼠肝组织中共检出47种表达水平差异较显著的基因,其中9种基因与脂代谢相关。创面脓毒症组大鼠这9种基因中表达上调的是与脂肪酸转运和活化功能相关的多种酶基因,表达下调的是与脂肪酸在线粒体内氧化供能相关的多种酶基因。结论烫伤大鼠创面脓毒症的发生引起肝脏组织中多种脂代谢相关基因表达水平改变,加重了烫伤后的脂代谢紊乱;初步判断脂代谢障碍的发生部位为线粒体。Objective To examine the expression profile of lipid metabolism-related genes in liver of scalded rats with wound sepsis, and to analyze its significance. Methods Sixty male Wistar rats with 30% TBSA full-thickness scald wound on the back were enrolled in the study and randomly divided into wound sepsis group （ n = 30） and control group （ n = 30, with scald）. Pseudomonas aeruginosa was inoculated to the wounds in sepsis group. Corresponding indices were determined to verify the diagnosis of wound sepsis. The rats were sacrificed and fresh liver tissues were obtained at 96 post-scald hours （PSH）. Total RNA of liver was isolated with Trizol and the different expression of lipid metabolism related genes in response to burn wound sepsis was assessed by DNA microarray. Results By comparing expression profile of the two groups, totally 47 genes were observed to be differentially expressed in rat hepatic tissues, among them 9 genes were related to lipid metabolism. Among them, those which were upregulated were genes in relation with transportation and activation of fatty acid, and those downrcgulated were genes related in providing energy for fatty acid oxidation in mitochondria. Conclusion The occurrence of wound sepsis in scalded rats can induce changes in the expression of hepatic lipid metabolism related genes in hepatic tissues, and aggravate the lipid metabolism disturbance that occur in mitochondria.

关 键 词：烧伤 脓毒症 肝 基因表达 脂代谢 

分 类 号：R644[医药卫生—外科学]