rhG-CSF对健康供者外周血和骨髓免疫特性影响的比较  

Effects of rhG-CSF Mobilization on Immunological Properties of Grafts from Peripheral Blood and Bone Marrow

作　　者：赵翔宇[1] 常英军[1,2] 黄晓军[1] 

机构地区：[1]北京大学人民医院北京大学血液病研究所,北京100044 [2]现在河北医科大学第三医院血液科

出　　处：《中国实验血液学杂志》2006年第4期787-790,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号30370591);卫生部基金(2003);教育部博士点基金(编号20020001086)资助项目

摘　　要：本研究探讨rhG-CSF对健康供者外周血采集物(G-PB)和骨髓采集物(G-BM)免疫学特性影响的异同。用MTT法和夹心酶联免疫复合物(ELISA)法检测G-PB和G-BM中T淋巴细胞增殖能力和IL-4、IFN-γ的分泌,并用流式细胞术测定两种移植物的T细胞亚群、树突状细胞(DC)亚群、单核细胞及共刺激分子CD28的表达。结果表明G-PB中淋巴细胞,CD3+、CD4+、CD8+T淋巴细胞,DC1、DC2以及单核细胞的含量,CD4/CD8的比值高于G-BM(P<0.001)。G-PB的T淋巴细胞增殖能力高于G-BM(P<0.05);每微升G-PB移植物中T淋巴细胞分泌细胞因子IFN-γ、IL-4的量均明显高于G-BM,且G-PB中IL-4/IFN-γ比值小于G-BM(P<0·001),DC2与T淋巴细胞的比值也低于G-BM(P<0.01);而G-PB中CD4+、CD8+细胞上CD28表达的百分比和总体表达均高于G-BM(P<0·001)。结论rhG-CSF体内应用诱导G-PB和G-BM产生的T细胞免疫低反应性是有差异的,而两者之间的差异是G-PB和G-BM移植后移植物抗宿主病(GVHD)发生率和程度不同的免疫学基础。This study was aimed to investigate the difference of immunological properties between recombination human granulocyte colony-stimulating factor （rhG-CSF） mobilized peripheral blood grafts （G-PB） and rhG-CSF primed bone marrow grafts （G-BM）. The lymphocyte proliferation ability and the quantities of interleukin-4 （IL-4） and interferon-γ （IFN-γ） secreted by T cells were determined by using MTT assays and sandwich ELISA; T cell subgroups, dendritic cells （DC）, monocytes and the expression of CD28 costimulatory molecules on T cells were determined by multicolor flow cytometry. The results showed that the absolute numbers of lymphocytes, monocytes, CD3^＋, CD4^＋ and CD8^＋ T cells as well as DC1 and DC2,the ratios of CD4/CD8 in G-PB were significantly higher than those in G-BM, respectively （P 〈 0.001 ）. T cell proliferation ability was significantly higher in G-PB than that in G-BM （P 〈 0.05 ）. The quantities of IFN-γ and IL-4 secreted by T cells per micromilter of G-PB was significantly higher than those of G- BM, the ratios of IL-4/IFN-γ were significantly lower in G-PB than that in G-BM （P 〈0.001 ）. As compared with G- BM, the ratio between DC2 and T-lymphocyte was significantly low in G-PB （P 〈0.01 ）, whereas the percentage and overall expression of CD28 on CD4^＋ and CD8^＋ cells were significantly high in G-PB （ P 〈 0.05）. It is concluded that T cell hyporesponsivenesses of G-PB and G-BM induced by rhG-CSF in vivo were confn-med to be different, and the difference of immunological properties between G-PB and G-BM may explain the lower incidence of GVHD and lower relapse after G-BM and G-PB transplantation respectively.

关 键 词：粒细胞集落刺激因子 外周血移植物 骨髓移植物 共刺激分子 免疫低反应性 

分 类 号：R457.7[医药卫生—治疗学] R392.4[医药卫生—临床医学]