mPEG修饰红细胞的动物实验  

Animal Trials for mPEG-Modified Red Blood Cells

作　　者：邱艳[1] 檀英霞[2] 李立立[2] 李素波[2] 吕秋霜[1] 查袆[1] 章扬培[2] 

机构地区：[1]北京红十字血液中心,北京100088 [2]军事医学科学院野战输血研究所,北京100850

出　　处：《中国实验血液学杂志》2006年第4期816-821,共6页Journal of Experimental Hematology

基　　金：国家973课题(编号2002CB713804);2001年北京市科技新星计划(编号200100775);北京市优秀人才培养专项经费(编号20042D0302049)

摘　　要：本研究采用异硫氰酸荧光素标记鼠、猕猴、猪和人的修饰与未修饰自体和异体红细胞(RBC)并给小鼠和猕猴实施输注,观察回输体内RBC24小时存活率的变化和差异。结果发现未修饰小鼠RBC在小鼠体内24小时存活率为74%,2.0mmol/LmPEG-SPA修饰的小鼠RBC在小鼠体内24小时存活率为45%,两者间有显著差异;未修饰人RBC在小鼠体内24小时存活率为8%,2.0mmol/LmPEG-SPA修饰人RBC在小鼠体内24小时存活率为5%,两者间无显著差异。修饰的猕猴RBC自体回输后24小时的存活率大于90%,而修饰的人RBC和猪RBC在给猕猴回输后2小时其存活率已降至零。结论RBC标记方法及不同种实验小鼠的选择对小鼠实验结果影响不大,但mPEG种类和浓度对鼠RBC寿命的有一定程度影响。用鼠RBC做自体回输来评价mPEG修饰人RBC的中mPEG是否影响人红细胞的寿命是不可靠的,修饰人RBC回输给小鼠可以作为评价修饰人RBC寿命和安全性的动物模型。大哺乳动物异种RBC输注很难观察到修饰RBC的存活率和寿命长短。适合评价修饰人RBC寿命和安全性的大动物模型有待于进一步探索。This study was aimed to investigate the survival rate and difference of transfused modified and ummodified RBC at 24 hours. The modified and unmodified RBC from mice, monkey, pig and human were labeled by using FITC, then these blood RBCs were transfused to homogeneous and heterogeneous animals. The result showed that 24 hour survival rate of unmodified mice RBC transfused to mice was 74%, while survival rate of 2.0 mmoL/L mPEG-SPA modified mice RBC transfused to mice was 45%, difference between them was significant. The 24 hour survived rate of unmodified human RBC transfused to mice was 8%, while 24 hours survival rate of 2.0 mmoL/L mPEG-SPA modified human RBC transfused to mice was 5% without statistical difference. The 24 hour survived rate of homogeneous transfusion of modified monkey RBC was 90%, while survival rate of modified human and pig RBC was zero on 24 hours after transfusion to monkey. It is concluded that RBC labeling methods and mice species are unrelated to 24 hours survival rate, but mPEG variety and concentration are related to mouse RBC life-span. It is incredible to use mouse RBC homogeneous transfusion result instead of human RBC to evaluate longevity and safety of modified human RBC. But modified human RBC transfused to mice can be a model to evaluate longevity of modified human RBC. It is very difficult to get the result about modified RBC life span by RBC transfusion among great heterogeneous mammal animals. So evaluation in large mammal animal models needs to be further studied.

关 键 词：mPEG—SPA 红细胞 输血 动物模型 

分 类 号：R331.141[医药卫生—人体生理学] R457.1[医药卫生—基础医学]