构建抗体微阵列过程中抗体固定的策略  

Strategies for Antibody Immobilization During the Fabrication of Antibody Microarrays

作　　者：张露[1] 刘必成[1] 

机构地区：[1]东南大学附属中大医院肾科肾脏病研究所,南京210009

出　　处：《生物医学工程学杂志》2006年第4期907-910,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30570476);卫生部科学研究基金资助项目(WKJ2004-2-006)

摘　　要：抗体微阵列作为蛋白组学研究的一个技术平台,可以高通量并行检测多种蛋白质。由于抗体结构的特殊性,将抗体固定于固相载体表面已成为构建抗体微阵列构成中的关键步骤。本文就近年来用于构建抗体微阵列的几种抗体固定的方式及其优缺点进行综述。As one of the proteomic technologies ,antibody microarrays can provide a powerful tool for the high-throughput parallel analysis of thousands of proteins in a single experiment. Because antibodies are chemically and structurally complex; immobilizing antibody on solid phase surface of substrates has been a key step during the development of antibody microarrays. This review will focus on the current strategies for antibody immobilization and discuss the advantages and disadvantages of these methods.

关 键 词：抗体微阵列 抗体 固定 

分 类 号：R392[医药卫生—免疫学]