金钠多对脂多糖所致急性肺损伤大鼠的保护作用  

Protective effect of Ginaton on lipopolysaccaride-induced acute lung injury in rats

作　　者：陆国华[1] 单仁飞[1] 周建英[1] 姚航平[1] 

机构地区：[1]浙江大学医学院附属第一医院呼吸内科,杭州310003

出　　处：《中华急诊医学杂志》2006年第8期711-714,共4页Chinese Journal of Emergency Medicine

基　　金：浙江省科技厅重点项目(011103717)

摘　　要：目的研究金钠多对脂多糖(LPS)所致急性肺损伤(ALI)的保护机制及治疗作用。方法采用尾静脉注射脂多糖,按5 mg/kg剂量注射,复制急性肺损伤大鼠模型。将63只Wistar品系大鼠随机分成3组,分别为空白对照组、脂多糖损伤组、金钠多治疗组。各组再随机分为3组,分别为尾静脉注射后2 h、6 h和10 h。利用酶联免疫吸附试验(ELISA)检测血清中可溶性ICAM-1(sICAM-1)水平,肺组织标本分别用免疫组化(定性)和蛋白印迹(定量)方法检测其NFkB核因子。结果尾静脉注射脂多糖可成功复制出急性肺损伤大鼠模型；治疗组血清sICAM-1水平明显高于空白对照组(P＜0．01),损伤组的血清sICAM-1水平明显高于治疗组和空白对照组(P＜0．01)；免疫组化和蛋白印迹实验证实,NF-kB核因子在各组中的阳性表达强度为：金钠多损伤组＞脂多糖治疗组＞空白对照组(P＜0．01)。结论脂多糖可诱导急性肺损伤,金钠多对脂多糖所致的急性肺损伤具有显著的保护作用,其机制可能是抑制了肺组织细胞NF-kB的活性。Objective To investigate the effects of Ginaton on acute lung injury （ALI） induced by lipepolysaccaride （LPS）. Methods The acute lung injury rat model was induced by receiving 5 mg/kg LPS injection in tail vein. A total of 63 Wistar rats were divide into 3 groups without caring sex. Saline control group, LPS treated group and Ginaton treated group. The samples of different groups were collected 2 h, 6 h, and 10 h after tail vein administration. Serum soluble cell ashesion molecule - 1 （sICAM - 1 ） was measured by ELISA, staining and Western blotting of NF-κB were performed on sections of lung specimens. Results The acute lung injury rat model was successfully induced by receiving LPS injection in tail vein. The sICAM - 1 levels increased more in Ginaton treated group than those in Saline control group （P 〈 0.01）, and the increase in LPS treated group were the highest. By immunohistochemical staining, the positive NF-κB cells in Ginaton treated group were much less than those in LPS treated group （ P 〈 0.01 ）, and in Saline control group were least （ P 〈 0.01 ）. The results of the Western - blot method were consistent with immunohistochemical method. Conclusion ALI could be induced by LPS, Ginaton showed a protective effect through probably inhibiting activation of NF-κB on LPS-induced-ALI in this animal model.

关 键 词：脂多糖 急性肺损伤 金钠多 核转录因子 

分 类 号：R285[医药卫生—中药学]