盐酸戊乙奎醚对腹腔感染脓毒症小鼠肺iNOS表达的影响  

Effect of penehycUdine hydrochloride on iNOS expression in lung in septic mice

作　　者：雷利荣[1] 王焱林[1] 贾宝辉[1] 詹佳[1] 王成夭[1] 

机构地区：[1]武汉大学中南医院麻醉科,武汉430071

出　　处：《中华急诊医学杂志》2006年第8期735-737,共3页Chinese Journal of Emergency Medicine

基　　金：湖北省卫生厅科研项目(2005GX2C61)

摘　　要：目的观察盐酸戊乙奎醚对腹腔感染脓毒症小鼠肺组织iNOS表达和小鼠生存率的影响。方法采用盲肠结扎穿孔(CLP)法复制腹腔感染脓毒症动物模型。小鼠分假CLP组、CLP组和PHC组,PHC组于CLP前1 h腹腔注射PHC 0．45 mg/kg。CLP后6 h取肺组织进行光镜和电镜检查,12 h取肺组织采用原位杂交法检测iNOS mRNA的表达,并观察三组小鼠24 h生存率。结果与CLP组相比,PHC组小鼠肺脏病理损伤明显减轻,肺组织iNOS mRNA表达显著降低(P＜0．05)。CLP组小鼠24 h生存率26．7%,PHC组小鼠24 h生存率70．0%明显提高(P＜0．05)。结论PHC能提高腹腔感染脓毒症小鼠24 h生存率,抑制肺组织iNOS mRNA的表达,减轻肺脏病理损伤。Objective To investigate the effect of penehyclidine hydrochloride （PHC） on iNOS expression in lung in septic mice. Methods Female KM mice were subjected to cecal ligation and puncture （CLP, a model of polymicrobial sepsis） or shame operation. Thirty-six KM mice weighing 20 - 25 g were randomly assigned into three groups： shame CLP group, CLP group and PHC group. Mice in PHC group were given PHC 0.45 mg/kg intraperitoneally at 1 h before CLP. Six hours after CLP, the lungs of each group were sampled for light and electronic microscopy. The expression of iNOS mRNA in lung tissue were detected by in situ hybridization. The survival rates were observed at 24 h after the operation. Results The CLP group was observed thickened alveolar sepia, as well as mitochondrial cristae swelling and mitochondrial vacuolation under electronic microscope. Emptied lamellar bodies could be also found. Histology of lung in PHC group had little changed. Expression of iNOS in lung in PHC group was significantly lower than that of the CLP group. At 24 h after CLP challenge, 70.0% of the PHC mice lived, remarkably increased compared with that of the CLP group （26.7%）, P 〈 0.05. Conclusion PHC had effective effect for increasing the survival rates of septic mice, inhibiting the expression of iNOS and reducing the severity of lung injury.

关 键 词：盐酸戊乙奎醚 腹腔感染 脓毒症 诱导型一氧化氮合酶 

分 类 号：R459.7[医药卫生—急诊医学]