用分子生物学技术检测人乳头瘤病毒并分型  

Using Biological Molecular Technique to Detect Human Papiliomavirus DNA and its Typing

作　　者：李正言[1] 刘淑英[1] 李昭[1] 刘静[1] 

机构地区：[1]天津医科大学总医院妇科,300052

出　　处：《天津医药》1996年第10期604-606,共3页Tianjin Medical Journal

摘　　要：应用PCR技术,取227例患者下生殖道赘生物分泌物或活组织进行HPV DNA检测。211例可疑HPV感染者,HPV阳性者121例(57.35％)174例次,其中48例次(27.59％)为18型,31例次(17.82％)为16型,95例次(54.60％)为11/6b型。另16例中宫颈癌7例,HPV18型者6例次,11/6b型3例次;外阴癌2例均为18型;卵巢癌2例均为18型;宫颈不典型增生1例为11/6b型。余4例HPV阴性均为与感染无关的盆腔疾病。The polymerase chain reaction(PCR)technique was used for detn-ting HPV 11/6b,16 and 18 in cytological samples obtained from the lower genital tract of 227 patients. Human papillomavirus (HPV) was detected positive in 121(57- 3%) of 211 patients with suspected condyloma acuminatum (CA). HPV DNA 18 was positive 48 times(27. 59%);HPV 16 was positive 31 times(17. 82%);HPV DNA ll/6b was positive 95 times(54. 60%). Sixteen cases other than gynecological diseases complicated by suspected CA including 7 cases of cervical carcinoma(HPV DNA18.6 times(ll/6b,3 times) t 2 cases of vulvar cancer(all of HPV DNA 18);2 cases of ovarian cancer(all of HPV DNA 18)and 1 case of cervical squamous cell dysplasia(HPV DNA 11/6b). The other 4 patients were all negetive for HPV DNA.

关 键 词：尖锐湿疣 人乳头瘤病毒 聚合酶链反应 分型 诊断 

分 类 号：R752.530.4[医药卫生—皮肤病学与性病学]