HIVgp120对胎鼠背根神经节神经元突起生长的影响  

The effects of HIVgp120 on cultured dorsal root ganglion neuronal growth

作　　者：刘花香[1] 李振中[2] 邢毅[2] 黄飞[2] 杜芳[2] 刘真[2] 陈淑妍[2] 王丽红[2] 

机构地区：[1]山东大学齐鲁医院风湿科,山东济南250012 [2]山东大学医学院解剖学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第7期654-657,共4页Journal of Shandong University:Health Sciences

基　　金：教育部留学回国人员科研启动基金资助课题;外教司留[2003]406号;山东省优秀中青年科学家奖励基金课题(02BS091);济南市科技发展计划重点项目(051075)。

摘　　要：目的:探讨人类免疫缺陷病毒(human immunodeficiency virus,HIV)gp120对体外培养的背根神经节神经元胞体和突起生长的影响。方法:分散培养的胎鼠背根神经节神经元用不同浓度的HIVgp120(125、250、500 pmol/L和1 nmol/L)处理,对分散培养的背根神经节神经元用MAP2免疫荧光标记后,用共聚焦激光扫描显微镜观察神经元胞体和突起的改变。结果:应用gp120后7 d,神经元突起的数目减少,长度变短,而神经元的胞体则没有明显变化。结论:HIV gp120可直接影响分散培养的背根神经节神经元突起的生长。Objective： To determine the effects of human immunodeficiency virus （HIV） gp120 on dissociated cttltured dorsal root ganglion（DRG） neuronal growth. Methods： Dissociated cultured rat embryo＇s DRG cells were treated with HIV gp120 at different concentration （125 pmol/L, 250 pmol/L, 500 pmol/L, 1 nmol/L, respectively） and then were processed for MAP2 immunofluorescent labeling, and furthermore the changes of neuronal cell body and neurites were observed with confocal laser scanning microscope. Results： The number and length of neurites were reduced after 7-day treatment with gp120 compared with control group, whereas the diameter of neuronal cell body was not obviously altered compared with control group. Conclusion： HIV gp120 may have direct influences on the outgrowth of neurites in dissociated DRG cell cultures.

关 键 词：HIV 神经节 脊 神经元 共聚焦激光扫描显微镜 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]