HPLC法和UV法测定复方五子颗粒剂中淫羊藿甙和总黄酮含量  

Detemination of Icariin and Flavonoids in Co-Wuzi Granule by HPLC and UV

作　　者：黄桂芳[1] 罗杰英[1] 

机构地区：[1]湖南中医学院药剂教研室,长沙410007

出　　处：《中草药》1996年第9期526-528,共3页Chinese Traditional and Herbal Drugs

摘　　要：应用HPLC法测定复方五子颗粒剂有效成分淫羊藿甙含量，其结果为0．050％，RSD0．72％，应用UV法（三波长）测定该颗粒剂有效部位淫羊藿总黄酮含量，结果为0．120％，RSD3．8％。HPLC法分离效果好，可用于复方单一成分含量测定，而UV法宜用于复方中有效部位含量测定。An HPLC method for the quantitative determination of icariin and an UV method for the determination of flavonoids in Co-Wuzi granule Preparation were developed. Results showed that the amount of icariin was 0. 050%, RSD=0. 72%, and the amount of flavonoids was 0. 120%, RSD=3. 8%. The HPLC method was very effective in the separation of active principles,suitable for the quantitative determination of single component in compounded TCM preparations,while the UV method may be used for the identification of active parts of TCM preparations.

关 键 词：淫羊藿甙 淫羊藿黄酮 高效液相色谱 五子颗粒剂 

分 类 号：R284.1[医药卫生—中药学]