5种笛鲷属鱼类的遗传多样性及分子标记  

Genetic Diversity and Molecular Markers of 5 Species of Snappers

作　　者：刘丽[1] 刘楚吾[1] 

机构地区：[1]广东海洋大学水产学院,湛江524025

出　　处：《农业生物技术学报》2006年第3期349-355,共7页Journal of Agricultural Biotechnology

基　　金：广东省科技计划项目重大专项(A3050202号);湛江市科技攻关项目(200017号)。

摘　　要：运用RAPD及SSR技术对笛鲷属的画眉笛鲷(Lutjanusvitta)、金带笛鲷(L.fulvus)、金焰笛鲷(L.fulviflamma)、千年笛鲷(L.sebae)和星点笛鲷(L.stellatus)等5种鱼的遗传多样性及其分子标记进行了研究。RAPD研究结果表明,5种鱼的平均多态性位点比率(P)依次为89.30%、86.70%、92.11%、86.47%和86.00%;种内两个体间平均遗传距离(D)分别为0.3431,0.2130,0.3121,0.1825和0.1775;平均遗传多样性指数(Hi)分别为0.1634、0.1095、0.1353、0.1022和0.1024。引物OPA8和OPP10的扩增产物中得到9个RAPD标记,分别为OPA8-413bp、OPA8-140bp、OPP10-418bp、OPA8-697bp、OPP10-526bp、OPA8-361bp、OPP10-449bp、OPA8-311bp和OPP10-599b,可鉴别5种鱼。SSR研究结果显示,5种鱼的有效等位基因数依次为1.9610、3.3793、3.2957、1.7893和3.6591;群体的杂合度(H)分别为0.332、0.462、0.593、0.367和0.676;多态信息含量(PIC)分别为0.302、0.438、0.554、0.332和0.641。在11个微卫星位点上得到6个微卫星标记,分别为13Prs229-115bp、4Lca43-212bp、4Lca43-240bp、13Prs229-288bp、19Prs275-156bp和7Lca91-118bp可用于鉴别5种鱼。In order to protect and develop the valuable fishes of snappers, genetic diversity and molecular markers of 5 species of snappers, Lutjanus vitta, L. fulvus, L. fulviflamma, L. sebae and L. stellatus, were detected using RAPD and SSR technique. The amplified products of genomic DNA in the 5 species of snappers by RAPD technique indicated that the polymorphic loci ratio （P） were 89.30%, 86.70%, 92.11%,86.47% and 86.00%, the mean genetic distances of intra-species （D） were 0.3431,0.2130,0.3121, 0.1825 and 0.1775, and the genetic diversity indexes of intra-species （Hi） were 0.1634,0.1095 ,0.1353, 0.1022 and 0.1024 respectively. Nine molecular markers, OPA8^-413bp、OPA8^-140bp、OPP10^-418bp、OPA8^-697bp、OPP10^-526bp、OPA8^-361bp、OPP10^-449bp、OPA8^-311b and OPP10^599b which could be used for identifying 5 species of snappers were found in the amplified products by primer of OPA8 and OPP10. The amplified products of genomic DNA in 5 species of snappers by SSR technique showed that effective numbers of allelic was 1.9610, 3.3793,3.2957, 1.7893 and 3.6591 respectively, average heterozygosities were 0.332,0.462,0.593,0.367 and 0.676, polymorphism information contents （PIC） were 0.302,0.438,0.554,0.332 and 0.641 respectively. Six molecular markers, 13 Prs229^-115bp、4 Lca43^-212bp、4 Lca43^-240bp、13 Prs229^-288bp、19 Prs275^-156bp and 7Lca91^-118bp which could be used for identifying 5 species of snappers were found in 11 loci ofmicrrosatellite.

关 键 词：遗传多样性 分子标记 RAPD SSR 画眉笛鲷 金带笛鲷 金焰笛鲷 千年笛鲷 星点笛鲷 

分 类 号：S965[农业科学—水产养殖]