转nsLTPs类抗菌蛋白基因烟草对赤星病和青枯病的抗病性分析  

Analysis of Disease Resistance of nsLTPs-like Antimicrobial Protein Gene Transgenic Tobacco against Brown Leaf Spot and Granville Blast

作　　者：王晓雯[1] 杨星勇[1] 李德谋[1] 桑贤春[1] 佘容[1] 裴炎[1] 

机构地区：[1]农业部生物技术与作物品质改良重点开放实验室西南大学生物技术研究中心,重庆400716

出　　处：《农业生物技术学报》2006年第3期365-370,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30270147和No.30370916)资助。

摘　　要：以烟草叶盘为受体,通过根癌农杆菌(Agrobacteriumtumefaciens)介导法将益母草(LeonurusjaponicusHoutt.)非专一性脂转移蛋白(nsLTPs)类抗菌蛋白基因Lj-L和相应改造基因Lj-S分别导入烟草(Nicotianatabacumcv.Xanthi.),并进行抗病性分析。GUS染色和PCR分子验证表明外源基因已整合进烟草基因组中。RT-PCR分析表明,外源基因能够在烟草中稳定转录。T0代离体抗性实验表明,外源基因对烟草赤星病(Alternariaalternata)的抗性已达极显著水平;T1代活体抗性实验表明,Lj-L和Lj-S基因对青枯病(Pseudomanassolanacearum)的抗性也均达显著水平。抗性结果比较发现,Lj-L基因的抗病性效果优于Lj-S基因,且离体和活体抗病结果较一致,但并不与基因的转录水平成正比,似乎存在一个阈值。Both Lj-L gene and its modified version Lj-S gene from Leonurus japonicus Houtt were transformed into tobacco （Nicotiana tabacum cv. Xanthi） mediated by Agrobacterium tumefaciens. GUS and PCR identification showed that foreign gene was integrated into tobacco genome. RT-PCR analysis revealed that foreign gene could transcript normally. Analysis of resistance to pathogenic fungus （Alternaria alternata） using detatched leaves indicated that disease indexes （DI） of T0-transgenic tobacco was remarkably decreased from 100% for wild type to 27.66% for Lj-L gene and 40.77% for Lj-S gene respectively. Similar results were obtained in the in vivo analyses of resistance to pathogenic bacterium Pseudomanas solanacearum. The results suggested that the nsLTPs-like protein gene Lj-L might have great potential in generating transgenic plants with broad-spectntm resistance to both fungal and bacterial pathogens.

关 键 词：非专一性脂转移蛋白(nsLTPs) 抗病 赤星病菌 青枯病菌 烟草 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]