西安地区荷斯坦奶牛群5个基因位点遗传多态性与泌乳性状的相关分析  

Associations between Genetic Polymorphisms at Five Loci and Milk Production Traits in Xi'an Population of Chinese Holstein

作　　者：张润锋[1] 陈宏[1] 雷初朝[1] 房兴堂[2] 孙维斌[1] 胡沈荣[1] 苏利红[1] 

机构地区：[1]西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,杨凌712100 [2]徐州师范大学细胞与分子生物学研究所,徐州221116

出　　处：《农业生物技术学报》2006年第3期329-333,共5页Journal of Agricultural Biotechnology

基　　金：农业部留学归国人员活动基金项目(No.1999-12D);江苏省高校自然科学基金(No.03KJD230216);陕西省自然科学基金(No.2004C122);西北农林科技大学拔尖人才支持计划项目;西北农林科技大学研究生教育创新计划项目(No.05YCH018)资助。

摘　　要：首次以IGFBP-3基因作为中国荷斯坦牛泌乳性状的候选基因,对中国荷斯坦奶牛群5个基因位点中4个多态基因位点与泌乳性状进行了相关分析。κ-cn、β-lg、β-lg5'侧翼区和IGFBP-3多态基因位点基因型与泌乳性状的最小二乘分析和多重比较表明,κ-cn基因位点基因型对所有的性状影响不显著;IGFBP-3BB型的305d泌乳量显著高出AA、AB型1054.17kg和838.15kg(P<0.05),AB型的乳蛋白率显著高于BB型(P<0.05);β-lgAA型对乳脂率与乳蛋白率的比值有正效应,AB型对乳蛋白率具有负效应;β-lg5'侧翼区AA型的305d泌乳量显著高于AB型(P<0.05),AA型的乳脂率显著高于BB型(P<0.05),BB型的乳蛋白率显著高于AB型(P<0.05)。The genotypes effects of four polymorphic loci on milk production traits of the Holstein cattles were analyzed using insulin-like growth factor binding protein 3 （IGFBP-3） gene as a candidate gene of milk production traits in Chinese Holstein cattle firstly, The effects of genotypes of κ-cn locus on the traits were not statistically significantly（P〉0,05）, At IGFBP-3 locus, 305 days milk yield of individuals with genotype BB was higher than that of individuals with genotype AA and AB for 1054.17 kg and 838.15 kg respectively（P〈0.05）, the protein percent of individuals with genotype AB was higher than that of individuals with genotype BB （P〈0.05）. Genotype AA of β-lg locus had significantly positive effect on fat/protein ratio, Genotype AB had significantly negative effect on the protein percent. At β-lg gene 5＇ flanking region, 305 days yield of individuals with genotype AA was higher than that of individuals with genotype AB（P〈0.05）, the fat percent of individuals with genotype AA was higher than that of individuals with genotype BB（P〈0.05）, but individuals with genotype BB had higher protein percent than individuals with genotyupe AB（P〈0.05）,

关 键 词：荷斯坦牛 乳蛋白基因 IGFBP-3基因 遗传多态性 泌乳性状 

分 类 号：S823.91[农业科学—畜牧学]