小白菜高效子叶离体植株再生体系的建立  

Development of high frequency plantlet regeneration system for in vitro culture of cotyledons of Chinese cabbage

作　　者：邓智年[1] 魏源文[1] 吕维莉[1] 李杨瑞[1] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《广西农业科学》2006年第4期350-354,共5页Guangxi Agricultural Sciences

摘　　要：以丰顺小白菜带柄子叶为外植体，研究了苗龄、不同激素配比、AgNO3浓度和抗生素、共培养时间对不定芽再生的影响。结果显示，子叶不定芽再生的最佳苗龄为5～9d；经过2d的预培养，在MS＋1．0mg／L TDZ＋0．1mg／LNAA＋7．5mg／LAgNO3＋0．5mg／LABA培养基中，带柄子叶不定芽再生率最高，为82．53％；在含有2．0mg／LIBA的1／2 MS0培养基中诱导生根，不定根形成率为100％。抗生素敏感性试验表明，可选择浓度为20mg／LHyg（潮霉素）作为最适合选择压，抑制农杆菌生长的抗生素选用500mg／LCb（羧卞青霉素）。不同浓度农杆菌菌液对子叶再生不定芽的影响不大，但共培养时间的长短却明显地影响不定芽的分化。The cotyledons with petiole from Fengshun Chinese cabbage were used as explants, and the effects of seedling age and different homone combinations, AgNO3 concentration and antibiotics, co-culture days with Agrobactium tumefaciens on the regeneration of adventitious bud of cotyledon were studied. The results indicated that the best seedling age to induce adventitious bud regeneration was 5-9 days, and the regeneration rate of cotyledon was the highest with 82.53% in medium MS＋1. 0mg/L TDZ＋0. 1mg/L NAA＋7. 5mg/L AgNO3＋0.5mg/L ABA after sub-cultured for two days; the formation rate of adventitious root was 100% in 1/2MS0 medium containing 2.0mg/L IBA for root induction. The results of antibiotics sensitivity experiments indicated that 20mg/L Hyg was the most appropriate for selective pressure and 500mg/L Cb was used to restrain the growth of Agrobactium tumefaciens. In addition, the differentiation of adventitious buds was evidently affected by co-culture days, but had no close relation with the concentration of Agrobactium tumefaciens.

关 键 词：小白菜 子叶 不定芽 农杆菌 

分 类 号：S634.3[农业科学—蔬菜学]