HPLC法测定强的松的血药浓度及其临床应用价值  

Quantitation of Serum Prednisone Concentration by High-Performance Liquid Chromatography

作　　者：夏运成[1] 张旭光[1] 李江[1] 李志兰[1] 刘伏友[1] 彭佑铭[1] 许向青[1] 姜文玲[1] 

机构地区：[1]中南大学湘雅二医院肾病研究所,湖南长沙410011

出　　处：《医学临床研究》2006年第8期1213-1215,1218,共4页Journal of Clinical Research

摘　　要：【目的】建立一种可靠、快捷的高效液相色谱（HPLC）法测定强的松（PS）血药浓度的方法，并探讨体内PS血药浓的临床应用价值。【方法】以乙酰苯胺为内标，采用HP1100系列高效液相色谱仪，分析柱HP Lichrosphere Ca柱（250mm×4mm，5um）；流动相为：甲醇：四氢呋喃：水=25：25：50（V／V），流速为1．0ml／min；检测波长为240nm；室温25℃。血清标本以甲醇沉淀蛋白。【结果】用5％高氯酸溶液分别配制5个不同浓度的SP标准液，制备标准曲线，结果强的松浓度与峰面积比值之间的线性关系良好，回归方程为Y=6．84×10-4X-39．4×10-4，相关系数为0．99993，回收率为98．70％，批间CV3．76％，批内CV2．25％。线性范围：10～500μg／ml；检测限：5mg／ml。【结论】色谱峰分离良好，无干扰。本法是一种可靠、便捷的检测血清PS浓度的方法，适用于临床与治疗肾病的血药浓度监测（TDM）及研究工作。[Objective]To develop a high-performance liquid chromatography method to determine the concentration of prednisone （PS） in human serum. [Methods]The HP1100 high performance liquid chromatographic （HPLC）system was used. The analytic column was a HP Lichrosphere C8 column （250 mm×4 mm, 5 um）. The mobile phase was methanol, tetrahydrofuran and water with the ratio 25： 25： 50, The flow rate was 1.0 ml/min, The detective UV wavement was 240 nm, The serum samples, added acetanilide as internal standard, deposited down the protein with methanol. [Results]The chromatography was good and not interfered by the components of the serum. Good Linearity was observed in 10～500 ug/L range for prednisone, the detection limit was 5 ng/mL, [Conclusion]This is an accurate and convenient method to determine the concentration of PS in serum.

关 键 词：泼尼松/血液 血液浓度 色谱法 高压液相 

分 类 号：R971.3[医药卫生—药品]