哈尔滨地区大麦黄矮病毒株系鉴定  

Identification of Strains of Barley Yellow Dwarf Virus in Harbin Area

作　　者：刘艳[1] 钱幼亭[1] 梁影屏[1] 苗洪芹[2] 

机构地区：[1]中国农业科学院植物保护研究所 [2]河北省农林科学院植物保护研究所

出　　处：《黑龙江农业科学》1996年第6期1-3,共3页Heilongjiang Agricultural Sciences

基　　金："八六三"资助项目

摘　　要：从黑龙江省哈尔滨地区田间采集典型小麦黄矮病株25份,用无毒麦二叉蚜(Shizaphis graminum)、麦长管蚜(Macrosiphum avenae)、禾谷缢管蚜(Rhopalosiphum-padi)传毒,经生物学分离并用BYDV—GAV、PAV、RPV、SGY四种抗血清进行酶联免疫吸附(即ELISA法)测定。结果表明:25份标样中由麦长管蚜(Ma)和麦二叉蚜(Sg)传播的有22份,与GAV抗血清有强反应,而与PAV、RPV、SGV抗血清无反应。表明GAV株系是哈尔滨地区大麦黄矮病毒的主要株系。Samples of 25 plants of wheat with symptoms of barley yellow dwarf were collected from Harbin area, Heilongjiang province. Each sample was tested by enzyme-linked immunosor-bent assays (ELISA) using antisera for GAV,PAV,RPV,SGV and identifed by a series of biological experiments involving comparative transmission by Schizaphis graminum, Rhopalosiphum Padi and Macrosiphum avenae. Results indicated that 22 of 25 smples were transmitted non-specifically by S. avenae and S. graminum, and reacted very strongly with GAV-globulin and not at all with PAV,RPV and SGV globulins. These results pionted that GAV is the major strain of Barley Yellow Dwarf Virus in Harbin area.

关 键 词：哈尔滨地区 大麦 黄矮病毒 酶联免疫吸附 鉴定 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]